JARID1B (KDM5B) Homogeneous Assay Kit BPS Bioscience

JARID1B (KDM5B) Homogeneous Assay Kit

3262.5 EUR

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quantity

Produktdetaljer

Katalognummer: 421 - 50512-2

Produktkategori: Företag och industri > Vetenskap och laboratorium

Leverantör:

BPS BioscienceGentaur

Storlek: 384 rxns.

50512-1

JARID1B (KDM5B) Homogeneous Assay Kit

The JARID1B Homogeneous Assay Kit is designed to measure JARID1B activity for screening and profiling applications. JARID1B, also known as PLU-1 and KDM5B, is a JumonjiC (JmjC) and ARID domain-containing histone lysine demethylase that exhibits demethylation activity toward H3-K4Me2 and H3-K4Me3. The JARID1B Homogeneous Assay Kit comes in a convenient AlphaLISA® format (Scheme 1), with biotinylated histone H3 peptide substrate, primary antibody, demethylase assay buffer, and purified JARID1B for 96 or 384 enzyme reactions. The key to the JARID1B Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JARID1B enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts. Our histone demethylase assays utilize highly specific antibodies that recognize demethylated products. First, a sample containing the enzyme is incubated with a biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

1425 €

50517

JARID1B (KDM5B) Chemiluminescent Assay Kit

The JARID1B Chemiluminescent Assay Kit is designed to measure JARID1B_x000D_activity for screening and profiling applications. JARID1B is a member of the Jumonji, AT-rich_x000D_interactive domain 1 (JARID1) histone demethylase protein family. It binds directly, with several_x000D_other proteins, to retinoblastoma protein which regulates cell proliferation. The key to the_x000D_JARID1B Chemiluminescent Assay Kit is a highly specific antibody that recognizes demethylated_x000D_substrate. With this kit, only three simple steps on a microtiter plate are required for detection of_x000D_demethylase activity. First, JARID1B enzyme is incubated with the methylated H3 peptide for one_x000D_hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary_x000D_antibody followed by the addition of the HRP substrate to produce chemiluminescence that can be_x000D_measured using a chemiluminescence reader.

1237.5 €

50514

JARID1B (KDM5B) TR-FRET Assay Kit

The JARID1B TR-FRET Assay Kit is designed to measure activity of JARID1B_x000D_in a homogeneous 384 reaction format. This FRET-based_x000D_assay requires no time-consuming washing steps, making it especially suitable for high_x000D_throughput screening applications. The JARID1B TR-FRET Assay Kit comes in a convenient_x000D_format, with histone H3 peptide substrate, a Eu-labeled antibody against methylated K4 residue_x000D_of Histone H3, demethylase assay buffer, TR-FRET detection buffer, dye-labeled acceptor, and_x000D_purified JARID1B for 384 enzyme reactions. The key to the JARID1B Assay Kit is a highly_x000D_specific antibody that recognizes demethylated substrate. With this kit, only three simple steps_x000D_on a microtiter plate are required for demethylase activity detection. First, a sample containing_x000D_JARID1B enzyme is incubated with the biotinylated substrate. Next, antibody is added. Finally,_x000D_dye-labeled acceptor is added followed by fluorescence reading.

3375 €

50510-2

JARID1A (KDM5A) Homogeneous Assay Kit

The JARID1A Homogeneous Assay Kit is designed to measure activity of the JARID1A for screening and profiling applications, using AlphaLISA® technology. The key to the JARID1A Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JARID1A enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

3262.5 €

50511-1

JARID1C (KDM5C) Homogeneous Assay Kit

The JARID1C Homogeneous Assay Kit is designed to measure JARID1C activity for screening and profiling applications. JARID1C, also known as SMCX (Smcy homolog, X-linked), KDM5C, or XE169, is a JumonjiC (JmjC) and ARID domain-containing histone lysine demethylase that exhibits demethylation activity toward H3-K4Me2 and H3-K4Me3. The JARID1C Homogeneous Assay Kit comes in a convenient AlphaLISA® format (Scheme 1), with biotinylated histone H3 peptide substrate, primary antibody, demethylase assay buffer, and purified JARID1C for 96 enzyme reactions. The key to the JARID1C Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JARID1C enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.Our histone demethylase assays utilize highly specific antibodies that recognize demethylated histone substrate. First, a sample containing the enzyme is incubated with a biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

1425 €

50511-2

JARID1C (KDM5C) Homogeneous Assay Kit

The JARID1C Homogeneous Assay Kit is designed to measure activity of the JARID1C for screening and profiling applications, using AlphaLISA® technology. The key to the JARID1C Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for demethylase activity detection. First, a sample containing JARID1C enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

3262.5 €

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