JARID1B (KDM5B) Homogeneous Assay Kit BPS Bioscience

JARID1B (KDM5B) Homogeneous Assay Kit

1425 EUR

In Stock

quantity

Produktdetaljer

Katalognummer: 421 - 50512-1

Produktkategori: Företag och industri > Vetenskap och laboratorium

Leverantör:

BPS BioscienceGentaur

Storlek: 96 rxns.

50512-2

JARID1B (KDM5B) Homogeneous Assay Kit

The JARID1B Homogeneous Assay Kit is designed to measure activity of the JARID1B for_x000D_screening and profiling applications. The key to the JARID1B Homogeneous Assay Kit is a highly specific antibody_x000D_that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate_x000D_are required for demethylase activity detection. First, a sample containing JARID1B enzyme is_x000D_incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added,_x000D_then donor beads, followed by reading the Alpha-counts.

3262.5 €

50517

JARID1B (KDM5B) Chemiluminescent Assay Kit

The JARID1B Chemiluminescent Assay Kit is designed to measure JARID1B_x000D_activity for screening and profiling applications. JARID1B is a member of the Jumonji, AT-rich_x000D_interactive domain 1 (JARID1) histone demethylase protein family. It binds directly, with several_x000D_other proteins, to retinoblastoma protein which regulates cell proliferation. The key to the_x000D_JARID1B Chemiluminescent Assay Kit is a highly specific antibody that recognizes demethylated_x000D_substrate. With this kit, only three simple steps on a microtiter plate are required for detection of_x000D_demethylase activity. First, JARID1B enzyme is incubated with the methylated H3 peptide for one_x000D_hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary_x000D_antibody followed by the addition of the HRP substrate to produce chemiluminescence that can be_x000D_measured using a chemiluminescence reader.

1237.5 €

50514

JARID1B (KDM5B) TR-FRET Assay Kit

The JARID1B TR-FRET Assay Kit is designed to measure activity of JARID1B_x000D_in a homogeneous 384 reaction format. This FRET-based_x000D_assay requires no time-consuming washing steps, making it especially suitable for high_x000D_throughput screening applications. The JARID1B TR-FRET Assay Kit comes in a convenient_x000D_format, with histone H3 peptide substrate, a Eu-labeled antibody against methylated K4 residue_x000D_of Histone H3, demethylase assay buffer, TR-FRET detection buffer, dye-labeled acceptor, and_x000D_purified JARID1B for 384 enzyme reactions. The key to the JARID1B Assay Kit is a highly_x000D_specific antibody that recognizes demethylated substrate. With this kit, only three simple steps_x000D_on a microtiter plate are required for demethylase activity detection. First, a sample containing_x000D_JARID1B enzyme is incubated with the biotinylated substrate. Next, antibody is added. Finally,_x000D_dye-labeled acceptor is added followed by fluorescence reading.

3375 €

50510-2

JARID1A (KDM5A) Homogeneous Assay Kit

The JARID1A Homogeneous Assay Kit is designed to measure activity of the JARID1A for screening and profiling applications, using AlphaLISA® technology. The key to the JARID1A Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JARID1A enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

3262.5 €

50511-1

JARID1C (KDM5C) Homogeneous Assay Kit

The JARID1C Homogeneous Assay Kit is designed to measure JARID1C activity for screening and profiling applications. JARID1C, also known as SMCX (Smcy homolog, X-linked), KDM5C, or XE169, is a JumonjiC (JmjC) and ARID domain-containing histone lysine demethylase that exhibits demethylation activity toward H3-K4Me2 and H3-K4Me3. The JARID1C Homogeneous Assay Kit comes in a convenient AlphaLISA® format (Scheme 1), with biotinylated histone H3 peptide substrate, primary antibody, demethylase assay buffer, and purified JARID1C for 96 enzyme reactions. The key to the JARID1C Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JARID1C enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.Our histone demethylase assays utilize highly specific antibodies that recognize demethylated histone substrate. First, a sample containing the enzyme is incubated with a biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

1425 €

50511-2

JARID1C (KDM5C) Homogeneous Assay Kit

The JARID1C Homogeneous Assay Kit is designed to measure activity of the JARID1C for screening and profiling applications, using AlphaLISA® technology. The key to the JARID1C Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for demethylase activity detection. First, a sample containing JARID1C enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

3262.5 €

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