JARID1C (KDM5C) Homogeneous Assay Kit

50511-1

JARID1C (KDM5C) Homogeneous Assay Kit

The JARID1C Homogeneous Assay Kit is designed to measure JARID1C activity for screening and profiling applications. JARID1C, also known as SMCX (Smcy homolog, X-linked), KDM5C, or XE169, is a JumonjiC (JmjC) and ARID domain-containing histone lysine demethylase that exhibits demethylation activity toward H3-K₄Me² and H3-K₄Me³. The JARID1C Homogeneous Assay Kit comes in a convenient AlphaLISA^® format (Scheme 1), with biotinylated histone H3 peptide substrate, primary antibody, demethylase assay buffer, and purified JARID1C for 96 enzyme reactions. The key to the JARID1C Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JARID1C enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.Our histone demethylase assays utilize highly specific antibodies that recognize demethylated histone substrate. First, a sample containing the enzyme is incubated with a biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

1425 €

50112

JARID1C(KDM5C), FLAG-tag

Human JARID1C, also known as SMCX (Smcy homolog, X-linked), KDM5C, or XE169, GenBank Accession No. NM_004187, full length, a.a. 2-1560(end), with N-terminal FLAG- and His-tags, MW= 181 kDa, expressed in Sf9 cells via a baculovirus expression system.

862.5 €

MBS388435-001mL

JARID1C/KDM5C antibody (pAb)

446.38 €

MBS9438985-01mLBiotin

JARID1C Conjugated Monoclonal Antibody

797 €

abx136123-100ul

Jumonji/ARID Domain-Containing Protein 1C (JARID1C) Antibody

892.9 €

abx136123-200ul

Jumonji/ARID Domain-Containing Protein 1C (JARID1C) Antibody

1231.48 €