JARID1B (KDM5B) TR-FRET Assay Kit
3375 EUR
In Stock
quantity
Produktdetaljer
Katalognummer: 421 - 50514
Produktkategori: Företag och industri > Vetenskap och laboratorium
Gentaur
Storlek: 384 rxns.
50512-1
JARID1B (KDM5B) Homogeneous Assay Kit
The JARID1B Homogeneous Assay Kit is designed to measure JARID1B activity for screening and profiling applications. JARID1B, also known as PLU-1 and KDM5B, is a JumonjiC (JmjC) and ARID domain-containing histone lysine demethylase that exhibits demethylation activity toward H3-K<sub>4</sub>Me<sup>2</sup> and H3-K<sub>4</sub>Me<sup>3</sup>. The JARID1B Homogeneous Assay Kit comes in a convenient AlphaLISA<sup>®</sup> format (Scheme 1), with biotinylated histone H3 peptide substrate, primary antibody, demethylase assay buffer, and purified JARID1B for 96 or 384 enzyme reactions. The key to the JARID1B Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JARID1B enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts. Our histone demethylase assays utilize highly specific antibodies that recognize demethylated products. First, a sample containing the enzyme is incubated with a biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.
1425 €
50512-2
JARID1B (KDM5B) Homogeneous Assay Kit
The JARID1B Homogeneous Assay Kit is designed to measure activity of the JARID1B for_x000D_screening and profiling applications. The key to the JARID1B Homogeneous Assay Kit is a highly specific antibody_x000D_that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate_x000D_are required for demethylase activity detection. First, a sample containing JARID1B enzyme is_x000D_incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added,_x000D_then donor beads, followed by reading the Alpha-counts.
3262.5 €
50517
JARID1B (KDM5B) Chemiluminescent Assay Kit
The JARID1B Chemiluminescent Assay Kit is designed to measure JARID1B_x000D_activity for screening and profiling applications. JARID1B is a member of the Jumonji, AT-rich_x000D_interactive domain 1 (JARID1) histone demethylase protein family. It binds directly, with several_x000D_other proteins, to retinoblastoma protein which regulates cell proliferation. The key to the_x000D_JARID1B Chemiluminescent Assay Kit is a highly specific antibody that recognizes demethylated_x000D_substrate. With this kit, only three simple steps on a microtiter plate are required for detection of_x000D_demethylase activity. First, JARID1B enzyme is incubated with the methylated H3 peptide for one_x000D_hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary_x000D_antibody followed by the addition of the HRP substrate to produce chemiluminescence that can be_x000D_measured using a chemiluminescence reader.
1237.5 €
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