JARID1B (KDM5B) Chemiluminescent Assay Kit
1237.5 EUR
In Stock
quantity
Produktdetaljer
Katalognummer: 421 - 50517
Produktkategori: Företag och industri > Vetenskap och laboratorium
Gentaur
Storlek: 96 rxns.
50512-1
JARID1B (KDM5B) Homogeneous Assay Kit
The JARID1B Homogeneous Assay Kit is designed to measure JARID1B activity for screening and profiling applications. JARID1B, also known as PLU-1 and KDM5B, is a JumonjiC (JmjC) and ARID domain-containing histone lysine demethylase that exhibits demethylation activity toward H3-K<sub>4</sub>Me<sup>2</sup> and H3-K<sub>4</sub>Me<sup>3</sup>. The JARID1B Homogeneous Assay Kit comes in a convenient AlphaLISA<sup>®</sup> format (Scheme 1), with biotinylated histone H3 peptide substrate, primary antibody, demethylase assay buffer, and purified JARID1B for 96 or 384 enzyme reactions. The key to the JARID1B Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JARID1B enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts. Our histone demethylase assays utilize highly specific antibodies that recognize demethylated products. First, a sample containing the enzyme is incubated with a biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.
1425 €
50512-2
JARID1B (KDM5B) Homogeneous Assay Kit
The JARID1B Homogeneous Assay Kit is designed to measure activity of the JARID1B for_x000D_screening and profiling applications. The key to the JARID1B Homogeneous Assay Kit is a highly specific antibody_x000D_that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate_x000D_are required for demethylase activity detection. First, a sample containing JARID1B enzyme is_x000D_incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added,_x000D_then donor beads, followed by reading the Alpha-counts.
3262.5 €
50514
JARID1B (KDM5B) TR-FRET Assay Kit
The JARID1B TR-FRET Assay Kit is designed to measure activity of JARID1B_x000D_in a homogeneous 384 reaction format. This FRET-based_x000D_assay requires no time-consuming washing steps, making it especially suitable for high_x000D_throughput screening applications. The JARID1B TR-FRET Assay Kit comes in a convenient_x000D_format, with histone H3 peptide substrate, a Eu-labeled antibody against methylated K4 residue_x000D_of Histone H3, demethylase assay buffer, TR-FRET detection buffer, dye-labeled acceptor, and_x000D_purified JARID1B for 384 enzyme reactions. The key to the JARID1B Assay Kit is a highly_x000D_specific antibody that recognizes demethylated substrate. With this kit, only three simple steps_x000D_on a microtiter plate are required for demethylase activity detection. First, a sample containing_x000D_JARID1B enzyme is incubated with the biotinylated substrate. Next, antibody is added. Finally,_x000D_dye-labeled acceptor is added followed by fluorescence reading.
3375 €
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