WWP1 Intrachain TR-FRET Assay Kit BPS Bioscience

WWP1 Intrachain TR-FRET Assay Kit

3007.5 EUR

In Stock

quantity

Produktdetaljer

Katalognummer: 421 - 78554

Produktkategori: Företag och industri > Vetenskap och laboratorium

Leverantör:

BPS BioscienceGentaur

Storlek: 384 reactions

78301

Cereblon intrachain TR-FRET Assay Kit

Covalent conjugation to ubiquitin (Ub) is one of the major post-translational modifications that regulates protein stability, function, and localization. Ubiquitination is the concerted action of three enzymes: a Ub-activating enzyme (E1), a Ub-conjugating enzyme (E2), and a Ub ligase (E3). The specificity and efficiency of ubiquitination are largely determined by the E3 enzyme, which directs the last step of the Ub-conjugating cascade by binding to both an E2~Ub conjugate and a substrate protein. This step ensures the transfer of Ub from E2~Ub to the substrate, leading to its mono- or poly-ubiquitination.The Cereblon (CRBN) protein via its interaction with the DNA damage-binding protein-1 (DDB1), Cullin 4 (Cul4A or Cul4B), and regulator of Cullins 1 (RoC1) forms the functional E3 Ub ligase complex. In this complex, Cereblon functions as a substrate receptor that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Cereblon complex is involved in many biological processes including cell proliferation and apoptosis. That is why it has been employed for targeted protein degradation in the treatment of cancer. Like most E3 ligases, Cereblon complex ubiquitinates itself and this auto-ubiquitination promotes its Ub ligase activity.The Cereblon intrachain TR-FRET Assay Kit is a sensitive high-throughput screening (HTS) TR-FRET Assay Kit, designed to measure Cereblon auto-ubiquitination activity in a homogeneous 384 reaction format. It utilizes a Europium cryptate-labeled Ub (donor) as well as Cy5-labeled Ub (acceptor) to complete the TR-FRET pairing. Since both the TR-FRET donor and acceptor are incorporated into poly-ubiquitin chains formed on Cereblon, this FRET-based assay requires no time-consuming washing steps, making it especially suitable for HTS applications as well as real-time kinetics analyses of polyubiquitination.

2775 €

78302

MDM2 intrachain TR-FRET Assay Kit

Covalent conjugation to ubiquitin (Ub) is one of the major post-translational modifications that regulates protein stability, function, and localization. Ubiquitination is the concerted action of three enzymes: a Ub-activating enzyme (E1), a Ub-conjugating enzyme (E2), and a Ub ligase (E3). The specificity and efficiency of ubiquitination are largely determined by the E3 enzyme, which directs the last step of the Ub-conjugating cascade by binding to both an E2~Ub conjugate and a substrate protein. This step ensures the transfer of Ub from E2~Ub to the substrate, leading to its mono- or poly-ubiquitination.The human Mouse double minute 2 homolog (MDM2) is an E3 Ub ligase and the master regulator of tumor suppressor proteins such as p53. Thus, high activity of MDM2 can promote tumor formation by targeting tumor suppressor proteins for proteasomal degradation, enabling cancer cell survival and proliferation. That is why MDM2 is an attractive potential drug target in cancer immunotherapy. Like most E3 ligases, MDM2 ubiquitinates itself and this auto-ubiquitination stimulates MDM2 Ub ligase activity.The MDM2 intrachain TR-FRET Assay Kit is a sensitive high-throughput screening (HTS) TR-FRET Assay Kit, designed to measure MDM2 auto-ubiquitination activity in a homogeneous 384 reaction format. It utilizes a Europium cryptate-labeled Ub (donor) as well as Cy5-labeled Ub (acceptor) to complete the TR-FRET pairing. Since both the TR-FRET donor and acceptor are incorporated into poly-ubiquitin chains formed on MDM2, this FRET-based assay requires no time-consuming washing steps, making it especially suitable for HTS applications as well as real-time kinetics analyses of polyubiquitination.

2325 €

78303

SMURF1 intrachain TR-FRET Assay Kit

Covalent conjugation to ubiquitin (Ub) is one of the major post-translational modifications that regulates protein stability, function, and localization. Ubiquitination is the concerted action of three enzymes: a Ub-activating enzyme (E1), a Ub-conjugating enzyme (E2), and a Ub ligase (E3). The specificity and efficiency of ubiquitination are largely determined by the E3 enzyme, which directs the last step of the Ub-conjugating cascade by binding to both an E2~Ub conjugate and a substrate protein. This step ensures the transfer of Ub from E2~Ub to the substrate, leading to its mono- or poly-ubiquitination.The SMAD ubiquitination regulatory factor 1 (SMURF1) is a HECT-type E3 Ub ligase that regulates TGF-β/BMP pathways via ubiquitination of key signal transducers (SMAD1, SMAD2, or SMAD5), or TGF-β receptor I. SMURFs play a critical role in cell-type specification, tissue and organ development by regulating planar cell polarity signaling and convergent extension. SMURFs can also accelerate tumor progression, invasion, and metastasis as they regulate ubiquitination and subsequent proteasomal degradation of tumor-suppressing proteins including p53 as well as various cell signaling proteins. That is why SMURF1 and especially its Ub ligase activity is an attractive potential drug target in cancer immunotherapy. Like most E3 ligases, SMURF1 ubiquitinates itself.The SMURF1 intrachain TR-FRET Assay Kit is a sensitive high-throughput screening (HTS) TR-FRET Assay Kit, designed to measure SMURF1 auto-ubiquitination activity in a homogeneous 384 reaction format. It utilizes a Europium cryptate-labeled Ub (donor) as well as Cy5-labeled Ub (acceptor) to complete the TR-FRET pairing. Since both the TR-FRET donor and acceptor are incorporated into poly-ubiquitin chains formed on SMURF1, this FRET-based assay requires no time-consuming washing steps, making it especially suitable for HTS applications as well as real-time kinetics analyses of polyubiquitination.

2430 €

50514

JARID1B (KDM5B) TR-FRET Assay Kit

The JARID1B TR-FRET Assay Kit is designed to measure activity of JARID1B_x000D_in a homogeneous 384 reaction format. This FRET-based_x000D_assay requires no time-consuming washing steps, making it especially suitable for high_x000D_throughput screening applications. The JARID1B TR-FRET Assay Kit comes in a convenient_x000D_format, with histone H3 peptide substrate, a Eu-labeled antibody against methylated K4 residue_x000D_of Histone H3, demethylase assay buffer, TR-FRET detection buffer, dye-labeled acceptor, and_x000D_purified JARID1B for 384 enzyme reactions. The key to the JARID1B Assay Kit is a highly_x000D_specific antibody that recognizes demethylated substrate. With this kit, only three simple steps_x000D_on a microtiter plate are required for demethylase activity detection. First, a sample containing_x000D_JARID1B enzyme is incubated with the biotinylated substrate. Next, antibody is added. Finally,_x000D_dye-labeled acceptor is added followed by fluorescence reading.

3375 €

52078

EZH2 (Y641N) TR-FRET Assay Kit

The EZH2 (Y641N) TR-FRET Assay Kit is designed to measure activity of the_x000D_mutant EZH2 complex [EZH2 (Y641N)/EED/SUZ12/RbAp48/AEBP] in a homogeneous 384_x000D_reaction format. This FRET-based assay requires no time-consuming washing steps, making it_x000D_especially suitable for high throughput screening applications. The key to the EZH2 (Y641N) Assay Kit is a_x000D_highly specific antibody that recognizes methylated Histone H3K27. With this kit, only three_x000D_simple steps on a microtiter plate are required for methyltransferase detection. First, Sadenosylmethionine_x000D_is incubated with a sample containing assay buffer and methyltransferase_x000D_enzyme for 4 hours. Next, antibody to methylated H3K27 is added. Finally, dye-labeled acceptor_x000D_is added followed by fluorescence detection.

2475 €

52680

EZH2(Y641F) TR-FRET Assay Kit

The EZH2 (Y641F) TR-FRET Assay Kit is designed to measure activity of the mutant EZH2 complex (EZH2 (Y641F)/EED/SUZ12/RbAp48/ AEBP) in a homogeneous 384 reaction format. This FRET-based assay requires no timeconsuming washing steps, making it especially suitable for high throughput screening applications. The EZH2 (Y641F) TR-FRET Assay Kit comes in a convenient format, with histone H3 peptide substrate, a Tb-labeled antibody against methylated K27 residue of Histone H3, S-adenosylmethionine, methyltransferase assay buffer, TR-FRET detection buffer, dye-labeled acceptor, and purified EZH2 (Y641F) complex for 384 enzyme reactions. The key to the EZH2 (Y641F) Assay Kit is a highly specific antibody that recognizes methylated Histone H3K27. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for 240 minutes. Next, antibody is added. Finally, dye-labeled acceptor is added followed by fluorescence reading.

2475 €

Håll dig uppdaterad! Visa tidigare publikationer

Anaplasmos hos hundar och katter – allt du behöver veta

By: Author , 2 Comment