Cereblon intrachain TR-FRET Assay Kit

79899

Cereblon Binding Assay Kit

The Cereblon Binding Assay Kit (FP) is designed for testing and profiling of Cereblon (CRBN) inhibitors using Fluorescence Polarization. The assay is a competitive binding assay, based on the binding of fluorescently-labeled Thalidomide, a CRBN binder, to purified recombinant CRBN._x000D_ CRBN is the substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. CRBN is a direct protein target for the immunomodulatory and antiproliferative activities of thalidomide analogues such as lenalidomide and pomalidomide._x000D_ The Cereblon Binding Assay Kit (FP) comes in a convenient 96-well format, with enough purified CRBN, Cy5-labeled Thalidomide, and assay buffer for 100 enzyme reactions. In addition, the kit includes the CRBN inhibitor Pomalidomide for use as an inhibitor control._x000D_<br />The key to the CRBN Assay Kit is the fluorescently-labeled Thalidomide. Using this kit, only one simple step on a microtiter plate is required for CRBN reactions. The Cy5-labeled Thalidomide is incubated with a sample containing CRBN to produce a change in fluorescent polarization that can be measured using a fluorescence reader.

1050 €

79881

Cereblon Ubiquitination Homogeneous Assay Kit

The Cereblon Ubiquitination Homogeneous Assay Kit is designed to measure Cereblon auto-ubiquitination activity in a homogeneous 384 reaction format. It utilizes biotin-labeled ubiquitin and proper A-screen beads to complete the pairing. This homogeneous assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications.

1380 €

LKU605506-96T

Cereblon (CRBN) Magnetic Luminex Assay Kit

759.6 €

DLR-CRBN-Mu

Mouse Cereblon (CRBN) ELISA Kit

tissue homogenates or other biological fluids.

862.5 €

DLR-CRBN-Mu-48T

Mouse Cereblon (CRBN) ELISA Kit

A sandwich quantitative ELISA assay kit for detection of Mouse Cereblon (CRBN) in samples from tissue homogenates or other biological fluids.

1098.6 €

DLR-CRBN-Mu-96T

Mouse Cereblon (CRBN) ELISA Kit

A sandwich quantitative ELISA assay kit for detection of Mouse Cereblon (CRBN) in samples from tissue homogenates or other biological fluids.

1388.4 €