EZH2 (Y641N) TR-FRET Assay Kit
2475 EUR
In Stock
quantity
Produktdetaljer
Katalognummer: 421 - 52078
Produktkategori: Företag och industri > Vetenskap och laboratorium
Gentaur
Storlek: 384 rxns.
52680
EZH2(Y641F) TR-FRET Assay Kit
The EZH2 (Y641F) TR-FRET Assay Kit is designed to measure activity of the mutant EZH2 complex (EZH2 (Y641F)/EED/SUZ12/RbAp48/ AEBP) in a homogeneous 384 reaction format. This FRET-based assay requires no timeconsuming washing steps, making it especially suitable for high throughput screening applications. The EZH2 (Y641F) TR-FRET Assay Kit comes in a convenient format, with histone H3 peptide substrate, a Tb-labeled antibody against methylated K27 residue of Histone H3, S-adenosylmethionine, methyltransferase assay buffer, TR-FRET detection buffer, dye-labeled acceptor, and purified EZH2 (Y641F) complex for 384 enzyme reactions. The key to the EZH2 (Y641F) Assay Kit is a highly specific antibody that recognizes methylated Histone H3K27. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for 240 minutes. Next, antibody is added. Finally, dye-labeled acceptor is added followed by fluorescence reading.
2475 €
79277
EZH2 (Y641N) Chemiluminescent Assay Kit
The EZH2 (Y641N) Chemiluminescent Assay Kit is designed to measure activity of the mutant EZH2 complex (EZH2 (Y641N)/EED/SUZ12/RbAp48/ AEBP) for screening and profiling purposes.
4275 €
52076
EZH2 (Y641N) Chemiluminescent Assay Kit
The EZH2 (Y641N) Chemiluminescent Assay Kit is designed to_x000D_measure activity of the mutant EZH2 complex (EZH2 (Y641N)/EED/SUZ12/RbAp48/_x000D_AEBP) for screening and profiling purposes. The key to the_x000D_EZH2 (Y641N) Assay Kit is a highly specific antibody that recognizes methylated_x000D_Histone H3K27. With this kit, only three simple steps on a microtiter plate are required_x000D_for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample_x000D_containing assay buffer and methyltransferase enzyme for one hour. Next, primary_x000D_antibody is added. Finally, the strips are treated with an HRP-labeled secondary_x000D_antibody followed by addition of the HRP substrate to produce chemiluminescence that_x000D_can then be measured using a chemiluminescence reader.
2475 €
55200
L3MBTL1 TR-FRET Assay Kit
The L3MBTL1 TR-FRET Assay Kit is designed to measure the_x000D_inhibition of L3MBTL1 binding to its substrate in a homogeneous 384 reaction format._x000D_This FRET-based assay requires no time-consuming washing steps, making it especially_x000D_suitable for high throughput screening applications. The assay procedure is_x000D_straightforward and simple; a sample containing terbium-labeled donor, dye-labeled_x000D_acceptor, L3MBTL1, substrate, and an inhibitor is incubated for sixty minutes. Then, the_x000D_fluorescence intensity is measured using a fluorescence reader.
952.5 €
60701
PDE4D3 TR-FRET Assay Kit
The PDE4D TR-FRET Assay Kit is designed for identification of inhibitors of PDE4D using TR-FRET (Time Resoled Fluorescence Resonance Energy Transfer) technology. Using this kit, only two simple steps on a microtiter plate are required for the PDE4D activity assay. First, the fluorescent-labeled cAMP is incubated with a sample containing PDE4D3 for 1 hour. Second, a binding agent and a terbium donor are added to the reaction mix and incubated for 1 hour. Then, fluorescence intensity can be measured using a fluorescence reader.
757.5 €
60704
PDE1B TR-FRET Assay Kit
The PDE1B TR-FRET Assay Kit is designed for identification of inhibitors of PDE1B using TR-FRET (Time Resolved Fluorescence Resonance Energy Transfer) technology. Using this kit, only two simple steps on a microtiter plate are required for the PDE1B activity assay. First, the fluorescent-labeled cAMP is incubated with a sample containing PDE1B for 1 hour. Second, a binding agent and a terbium donor are added to the reaction mix and incubated for 30 minutes. Then, fluorescence intensity can be measured using a fluorescence reader.
757.5 €
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