EZH2 (Y641N) Chemiluminescent Assay Kit

52076

EZH2 (Y641N) Chemiluminescent Assay Kit

The EZH2 (Y641N) Chemiluminescent Assay Kit is designed to_x000D_measure activity of the mutant EZH2 complex (EZH2 (Y641N)/EED/SUZ12/RbAp48/_x000D_AEBP) for screening and profiling purposes. The key to the_x000D_EZH2 (Y641N) Assay Kit is a highly specific antibody that recognizes methylated_x000D_Histone H3K27. With this kit, only three simple steps on a microtiter plate are required_x000D_for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample_x000D_containing assay buffer and methyltransferase enzyme for one hour. Next, primary_x000D_antibody is added. Finally, the strips are treated with an HRP-labeled secondary_x000D_antibody followed by addition of the HRP substrate to produce chemiluminescence that_x000D_can then be measured using a chemiluminescence reader.

2475 €

52078

EZH2 (Y641N) TR-FRET Assay Kit

The EZH2 (Y641N) TR-FRET Assay Kit is designed to measure activity of the_x000D_mutant EZH2 complex [EZH2 (Y641N)/EED/SUZ12/RbAp48/AEBP] in a homogeneous 384_x000D_reaction format. This FRET-based assay requires no time-consuming washing steps, making it_x000D_especially suitable for high throughput screening applications. The key to the EZH2 (Y641N) Assay Kit is a_x000D_highly specific antibody that recognizes methylated Histone H3K27. With this kit, only three_x000D_simple steps on a microtiter plate are required for methyltransferase detection. First, Sadenosylmethionine_x000D_is incubated with a sample containing assay buffer and methyltransferase_x000D_enzyme for 4 hours. Next, antibody to methylated H3K27 is added. Finally, dye-labeled acceptor_x000D_is added followed by fluorescence detection.

2475 €

MBS515498-002mg

EZH2 (Y641N) Protein

675.88 €

MBS8533475-01mL

EZH2

624.88 €

MBS8533475-01mLAF405L

EZH2

905.38 €

MBS8533475-01mLAF405S

EZH2

905.38 €