UBCH5b TR-FRET Assay Kit BPS Bioscience

UBCH5b TR-FRET Assay Kit

1380 EUR

In Stock

quantity

Produktdetaljer

Katalognummer: 421 - 79896

Produktkategori: Företag och industri > Vetenskap och laboratorium

Leverantör:

UBCH13 is a noncanonical ubiquitin conjugating enzyme (E2) that has been implicated in a variety of cellular signaling processes due to its ability to catalyze the formation of lysine 63-linked polyubiquitin chains on various substrates. In particular, UBCH13 is required for signaling by a variety of receptors important in immune regulation, making it a candidate target for inflammatory diseases. UBCH13 is also critical for double-strand DNA repair and thus a potential radiosensitizer and chemosensitizer target for oncology._x000D_The UBCH13 TR-FRET Assay Kit is designed to measure UBCH13 ubiquitination activity in a homogeneous 384 reaction format. It utilizes biotin-labeled ubiquitin and a terbium-labeled anti-His antibody to complete the TR-FRET pairing. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications.

1755 €

79900

UBCH5a TR-FRET Assay Kit

UBCH5a (UBE2D1) is an E2 conjugating ubiquitin protein that functions in the ubiquitination of the tumor-suppressor protein p53. It is a promising drug target in cancer immunotherapy. The UBCH5a TR-FRET Assay Kit is designed to measure ubiquitination activity in a homogeneous 384 reaction format. It utilizes biotin-labeled Ubiquitin and a terbium-labeled antibody recognizing the His-tagged UBCH5a protein to complete the TR-FRET pairing. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications.

1380 €

79901

UBCH5c TR-FRET Assay Kit

UBCH5c (UBE2D3) is an E2 conjugating ubiquitin protein that functions in the ubiquitination of the tumor-suppressor protein p53. It is a promising drug target in cancer immunotherapy. The UBCH5c TR-FRET Assay Kit is designed to measure ubiquitination activity in a homogeneous 384 reaction format. It utilizes biotin-labeled Ubiquitin and a terbium-labeled antibody recognizing the His-tagged UBCH5c protein to complete the TR-FRET pairing. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications.

1380 €

50514

JARID1B (KDM5B) TR-FRET Assay Kit

The JARID1B TR-FRET Assay Kit is designed to measure activity of JARID1B_x000D_in a homogeneous 384 reaction format. This FRET-based_x000D_assay requires no time-consuming washing steps, making it especially suitable for high_x000D_throughput screening applications. The JARID1B TR-FRET Assay Kit comes in a convenient_x000D_format, with histone H3 peptide substrate, a Eu-labeled antibody against methylated K4 residue_x000D_of Histone H3, demethylase assay buffer, TR-FRET detection buffer, dye-labeled acceptor, and_x000D_purified JARID1B for 384 enzyme reactions. The key to the JARID1B Assay Kit is a highly_x000D_specific antibody that recognizes demethylated substrate. With this kit, only three simple steps_x000D_on a microtiter plate are required for demethylase activity detection. First, a sample containing_x000D_JARID1B enzyme is incubated with the biotinylated substrate. Next, antibody is added. Finally,_x000D_dye-labeled acceptor is added followed by fluorescence reading.

3375 €

52078

EZH2 (Y641N) TR-FRET Assay Kit

The EZH2 (Y641N) TR-FRET Assay Kit is designed to measure activity of the_x000D_mutant EZH2 complex [EZH2 (Y641N)/EED/SUZ12/RbAp48/AEBP] in a homogeneous 384_x000D_reaction format. This FRET-based assay requires no time-consuming washing steps, making it_x000D_especially suitable for high throughput screening applications. The key to the EZH2 (Y641N) Assay Kit is a_x000D_highly specific antibody that recognizes methylated Histone H3K27. With this kit, only three_x000D_simple steps on a microtiter plate are required for methyltransferase detection. First, Sadenosylmethionine_x000D_is incubated with a sample containing assay buffer and methyltransferase_x000D_enzyme for 4 hours. Next, antibody to methylated H3K27 is added. Finally, dye-labeled acceptor_x000D_is added followed by fluorescence detection.

2475 €

52680

EZH2(Y641F) TR-FRET Assay Kit

The EZH2 (Y641F) TR-FRET Assay Kit is designed to measure activity of the mutant EZH2 complex (EZH2 (Y641F)/EED/SUZ12/RbAp48/ AEBP) in a homogeneous 384 reaction format. This FRET-based assay requires no timeconsuming washing steps, making it especially suitable for high throughput screening applications. The EZH2 (Y641F) TR-FRET Assay Kit comes in a convenient format, with histone H3 peptide substrate, a Tb-labeled antibody against methylated K27 residue of Histone H3, S-adenosylmethionine, methyltransferase assay buffer, TR-FRET detection buffer, dye-labeled acceptor, and purified EZH2 (Y641F) complex for 384 enzyme reactions. The key to the EZH2 (Y641F) Assay Kit is a highly specific antibody that recognizes methylated Histone H3K27. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for 240 minutes. Next, antibody is added. Finally, dye-labeled acceptor is added followed by fluorescence reading.

2475 €

Håll dig uppdaterad! Visa tidigare publikationer

Anaplasmos hos hundar och katter – allt du behöver veta

By: Author , 2 Comment