HUAECs are pooled cells isolated from normal human umbilical artery. RFP-HUAECs were selected from HUAECs infected with RFP-expressing lentiviruses with Zyocin and the cells are shipped in frozen vials (the cells are provided @ passage 3). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels 20 when cultured following the detailed protocol.