AcceGen

Showing Products 1-30 of 51 Result

ABC-FC0001

GFP-Human Umbilical Vein Endothelial Cells

HUVECs were infected with GFP- expressing Lentiviral particles @ passage 1. Puromycin resistant GFP-HUVECs were selected and shipped in frozen vials (cells are provided @ passage 3). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum of 18 population doubling levels when cultured following the protocols described below.

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ABC-FC0002

Mitochondria GFP Tag Human Umbilical Vein Endothelial Cells

HUVECs were infected with Lentiviral particles expressing GFP-Tagged Cytochrome C oxidase VIII @ passage 1. Puromycin resistant Mitochondria-GFP-HUVECs were selected and shipped in frozen vials (cells are provided @ passage 3). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum of 18 population doubling levels when cultured following the protocols described below).

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ABC-FC0003

Plasma Membrane GFP Tag Human Umbilical Vein Endothelial Cells

HUVECs were infected with Lentiviral particles expressing GFP-Tagged neuromodulin @ passage 1. Puromycin resistant PM-GFP-HUVECs were selected and shipped in frozen vials (cells are provided @ passage 3). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum of 18 population doubling levels when cultured following the protocols described below).

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ABC-FC0004

RFP-Human Umbilical Vein Endothelial Cells

HUVECs were infected with RFP- expressing Lentiviral particles @ passage 1. Zeocin resistant RPF-HUVECs were selected and shipped in frozen vials (cells are provided @ passage 3). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum of 18 population doubling levels when cultured following the protocols described below).

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ABC-FC0005

GFP-Human Brain Microvascular Endothelial Cells

GFP-HBMVECs cells were selected from HBMVECs resistant to puromycin after infected with GFP expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 4-5). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels 15 when cultured following the detailed protocol described below).

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ABC-FC0006

GFP-Human Dermal Lymphatic Microvascular Endothelial Cells

GFP-HDLMVECs cells were selected from HGLMVECs resistant to puromycin after infected with GFP expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 3). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels 15 when cultured following the detailed protocol described below.

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ABC-FC0007

GFP-Human Glomerular Microvascular Endothelial Cells

GFP-HGMVECs were selected from HGMVECs resistant to puromycin after infected with GFP expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 4-5). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels 18 when cultured following the detailed protocol described below.

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ABC-FC0008

RFP-Human Glomerular Microvascular Endothelial Cells

RFP-HGMVECs were selected from HGMVECs resistant to Zeocin after infected with RFP expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 4-5). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels 18 when cultured following the detailed protocol described below.

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ABC-FC0009

GFP-Human Dermal Microvascular Endothelial Cells

GFP-HDMVECs were selected from HDMVECs resistant to puromycin after infected with GFP expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 3). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels 18 when cultured following the detailed protocol described below.

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ABC-FC0010

GFP-Human Aortic Artery Endothelial Cells

GFP-HAAECs were selected from HAAECs resistant to puromycin after infected with GFP expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 3). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels 14 when cultured following the detailed protocol described below.

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ABC-FC0011

GFP-Human Coronary Artery Endothelial Cells

GFP-HCAECs were selected from HCAECs resistant to puromycin after infected with GFP expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 5). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels 14 when cultured following the detailed protocol described below.

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ABC-FC0012

GFP-Human Pancreatic Microvascular Endothelial Cells

GFP-HPaMVECs were selected from HPaMVECs resistant to puromycin after infected with GFP expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 5). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels 14 when cultured following the detailed protocol described below.

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ABC-FC0013

GFP-Human Retinal Microvascular Endothelial Cells

GFP-HRMVECs were selected from HRMVECs resistant to puromycin after infected with GFP expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 5). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels 16 when cultured following the detailed protocol described below.

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ABC-FC0014

RFP-Human Retinal Microvascular Endothelial Cells

RFP-HRMVECs were selected from HRMVECs resistant to Zeocin after infected with RFP expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 5). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels 16 when cultured following the detailed protocol described below.

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ABC-FC0015

GFP-Liver Sinusoidal Microvascular Endothelial Cells

GFP-HLiSMVECs were selected from HRMVECs resistant to puromycin after infected with GFP expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 4-5). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels 16 when cultured following the detailed protocol described below.

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ABC-FC0016

RFP-Liver Sinusoidal Microvascular Endothelial Cells

RFP-HLiSMVECs were selected from HRMVECs resistant to Zeocin after infected with RFP expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 4-5). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels 16 when cultured following the detailed protocol described below.

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ABC-FC0017

GFP-Human Internal Mammary Artery Endothelial Cells

GFP-HIMAECs were selected from HIMAECs resistant to puromycin after infected with GFP expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 4-5). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels 16 when cultured following the detailed protocol described below.

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ABC-FC0018

GFP-Human Saphenous Vein Endothelial Cells

GFP-HSVECs were selected from HSVECs resistant to puromycin after infected with GFP expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 4-5). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels 16 when cultured following the detailed protocol described below.

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ABC-FC0019

RFP-Human Saphenous Vein Endothelial Cells

RFP-HSVECs were selected from HSVECs resistant to Zeocin after infected with RFP expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 4-5). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels 16 when cultured following the detailed protocol described below.

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ABC-FC0020

GFP-Human Pulmonary Microvascular Endothelial Cells

GFP-HPuMVECS were selected from HPlMVECs resistant to puromycin after infected with GFP expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 3-4). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels 10 when cultured following the detailed protocol described below.

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ABC-FC0021

GFP-Human Umbilical Artery Endothelial Cells

HUAECs are pooled cells isolated from normal human umbilical artery. GFP-HUAECs were selected from HUAECs infected with GFP-expressing lentiviruse with Puromycin and the cells are shipped in frozen vials (the cells are provided @ passage 3). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels 20 when cultured following the detailed protocol.

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ABC-FC0022

RFP-Human Umbilical Artery Endothelial Cells

HUAECs are pooled cells isolated from normal human umbilical artery. RFP-HUAECs were selected from HUAECs infected with RFP-expressing lentiviruses with Zyocin and the cells are shipped in frozen vials (the cells are provided @ passage 3). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels 20 when cultured following the detailed protocol.

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ABC-FC0023

Human VEGFR2 shRNA Stable Transfected GFP-HUVECs

HUVECs were infected with Lentiviral particles expressing human VEGFR2 specific shRNAs and GFP @ passage 1. Puromycin resistant HUVECs-shRNAVR2-GFP cells were selected and shipped in frozen vials (cells are provided @ passage 3). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum of 18 population doubling levels when cultured following the protocols described below).

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ABC-FC0024

GFP-Human Dermal Fibroblasts-Adult

HNDFCs-ad cells are isolated from normal adult skin tissue samples and transfected with GFP-Lentiviral particles at passage one. Puromycin resistant GFP-HNDFCs-Ad are selected and shipped in frozen vial (the cell are provided @passage 3). Fibroblast Growth medium is recommended for cell culture and these cells have an average minimum population doubling levels 14 when cultured following the detailed protocol described below.

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ABC-FC0025

RFP-Human Dermal Fibroblasts-Adult

HNDFCs-ad cells are isolated from normal adult skin tissue samples and transfected with RFP-Lentiviral particles at passage one. Zeocin resistant RFP-HNDFCs-ad are selected and shipped in frozen vial (the cell are provided @passage 3). Fibroblast Growth medium is recommended for cell culture and these cells have an average minimum population doubling levels 14 when cultured following the detailed protocol described below).

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ABC-FC0026

GFP-Human Dermal Fibroblasts-Neonatal

HNDFCs-Neo cells are isolated from normal neonatal foreskin tissue samples and transfected with GFP-Lentiviral particles at passage one. Puromycin resistant GPF-HNDFCs-Neo are selected and shipped in frozen vial (the cell are provided @passage 3). Fibroblast Growth medium is recommended for cell culture and these cells have an average minimum population doubling levels 14 when cultured following the detailed protocol described below.

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ABC-FC0027

GFP and Luciferase Expressing Human Neonatal Dermal Fibroblasts

HNDFCs-Neo cells are isolated from normal neonatal foreskin tissue samples and transfected with GFP and Luciferase expressing Lentiviral particles at passage one. Puromycin resistant GFP-Luc-HNDFCs-Neo are selected and shipped in frozen vial (the cell are provided @passage 3). Fibroblast Growth medium is recommended for cell culture and these cells have an average minimum population doubling levels 14 when cultured following the detailed protocol described below.

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ABC-FC0028

RFP-Human Dermal Fibroblasts-Neonatal

HNDFCs-Neo cells are isolated from normal neonatal foreskin tissue samples and transfected with RFP-Lentiviral particles at passage one. Zeocin resistant RFP-HNDFCs-Neo are selected and shipped in frozen vial (the cell are provided @passage 3). Fibroblast Growth medium is recommended for cell culture and these cells have an average minimum population doubling levels 14 when cultured following the detailed protocol described below.

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ABC-FC0031

GFP-Human Retinal Microvascular Pericytes

HRMPCs are initiated by elutriation from dissociated normal human retinal tissue and transfected with GFP-Lentiviral particles at passage 2. Puromycin resistant GPF-HRMPCs were selected and shipped in frozen vials (the cells are provided @ passage 4-5). Pericytes growth medium is recommended for cell culture and these cells have an average population doubling capacity 6 when cultured following the detailed protocol described below.

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ABC-FC0032

RFP-Human Retinal Microvascular Pericytes

HRMPCs are initiated by elutriation from dissociated normal human retinal tissue and transfected with RFP-Lentiviral particles at passage 2. Zeocin resistant RPF-HRMPCs were selected and shipped in frozen vials (the cells are provided @ passage 4-5). Pericytes growth medium is recommended for cell culture and these cells have an average population doubling capacity 6 when cultured following the detailed protocol described below.

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ABC-FC0033

GFP-Human Aortic Smooth Muscle Cells

GFP-HuASMCs are puromycin resistant cells selected from HuASMCs isolated from normal human aorta, after infected with GFP expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 3-4). Smooth Muscle Cells (SMCs) Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels 8 (2-3 passages) when cultured following the detailed protocol described below.

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ABC-FC0034

RFP-Human Aortic Smooth Muscle Cells

RFP-HuASMCs are Zeocin resistant cells selected from HuASMCs isolated from normal human aorta, after infected with RFP expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 3-4). Smooth Muscle Cells (SMCs) Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels 8 (2-3 passages) when cultured following the detailed protocol described below.

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ABC-FC0035

GFP-Human Placental Microvascular Pericytes

GFP-HPlMVPCs are Puromycin resistant HPlMVPCs isolated from normal human placental tissue, after infected GFP expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 3). Pericyte Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels 12 when cultured following the detailed protocol described below.

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ABC-FC0036

GFP-Human Brain Microvascular Pericytes

HBMVPCs are isolated from normal human brain cortical tissue. GFP-HBMVPCs are selected from puromycin resistant HBMVPCs after infected with GFP Expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 5). Pericyte Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels 10 when cultured following the detailed protocol described below.

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ABC-FC0037

RFP-Human Brain Microvascular Pericytes

HBMVPCs are isolated from normal human brain cortical tissue. RFP-HBMVPCs are selected from Zeocin resistant HBMVPCs after infected with RFP Expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 5). Pericyte Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels 10 when cultured following the detailed protocol described below.

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ABC-FC0038

GFP-Human Brain Astrocytes

HBAs are isolated from normal human brain cortical tissue. GFP-HBAs are selected from puromycin resistant HBAs after infected with GFP Expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 3). Astrocytes Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels 8 when cultured following the detailed protocol described below.

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ABC-FC0039

RFP-Human Brain Astrocyte

HBAs are isolated from normal human brain cortical tissue. RFP-HBAs are selected from Zeocin resistant HBAs after infected with RFP expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 3). Astrocytes Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels 8 when cultured following the detailed protocol described below.

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ABC-FC0040

GFP-Human Lung Fibroblasts-Adults

HLFCs-Ad are isolated from normal human lung tissue(adult) samples and GFP-HLFCs-Ad cells are selected by puromycin after infection HLFCs-Ad with lentiviruses expressing GFP and shipped in frozen vial (the cells are provided @ passage 2). Universal Growth Medium is recommended for cell culture and these cells have an average minimum population doubling levels 10 when cultured following the detailed protocol described below.

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ABC-FC0041

GFP-Human Liver Stellate Cells

HLiStCs are initiated by hepatic artery perfusion digestion with collagenase. HLiStCs are separated from the mixture of hepatic sinusoidal MV endothelial cells and Kupffer cells in the digestion solution. GFP-HLiStCs are selected from Puromycin resistant HLiStCs after infected with GFP expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 3). Stellate-Growth medium is recommended for cell culture and these cells have a minimum average population doubling capacity 8 when cultured following the detailed protocol described below.

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ABC-FC0042

RFP-Human Liver Stellate Cells

HLiStCs are initiated by hepatic artery perfusion digestion with collagenase. HLiStCs are separated from the mixture of hepatic sinusoidal MV endothelial cells and Kupffer cells in the digestion solution. RFP-HLiStCs are selected from Zeocin resistant HLiStCs after infected with RFP expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 3). Stellate-Growth medium is recommended for cell culture and these cells have a minimum average population doubling capacity 8 when cultured following the detailed protocol described below.

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ABC-FC0044

HighQC™ RFP-Human Bone Marrow-Derived Mesenchymal Stem Cell

RFP-HBMMSCs are selected from puromycin resistant HBMMSCs after transfected with RFP expressing lentiviral particles. RFP-HBMMSCs are supplied in cryovials at passage 3-4. Each lot of RFP-HBMMSCs is tested to ensure the cells can be passaged at least for three times (i.e., approximately 9 to 10 population doublings) after thaw. RFP-HBMMSCs should be maintained in complete growth media (Mesenchymal Stem Cell Growth Medium (MSCGM)) following the detailed protocol described below.

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ABC-FC0045

RFP-Human Neuron Precursor Cells

HNPCs are initiated by dissecting fetal brain tissue and digestion with collagenase. RFP expressing HNPCs (RFP-HNPCs) are selected from RFP expressing lentiviruses infected HNPCs. RFP-HNPCs are offered in frozen vial format (the cells are provided @ passage 2). RFP-HNPCs growth medium is recommended for cell culture and these cells have a minimum average population doubling capacity 6 when cultured following the detailed protocol described below.

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ABC-FC0046

RFP-Human Dopaminergic Neuron Cells

Human dopaminergic neuronal cells are isolated from fetal brain tissue obtained from agencies authorized to procure and distribute tissues for research. Dopaminergic neuronal cells are selected in tyrosine free medium supplemented with a mixture of growth factors. Actively growing population of cells are tested for tyrosine hydroxylase (TH) expression by immunocytochemistry. RFP-Expressing Human dopaminergic neuronal cells (RFP-HDaNCs) are selected by puromycin after HDaNCs are infected with lentiviruses expressing RFP. Cells are provided with more than 5x10[5] cells/ vial at passage 2. The cells are grown in regular tissue culture dishes and subcultured at a split ratio of 1 to 2 at confluence. We recommend not to use the cells beyond 4 passages. There may be a significant decrease in the number of tyrosine hydroxylase positive cells after 4 passages as they are not maintained in selective medium to allow the growth of only cells expressing tyrosine hydroxylase.

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ABC-FC0047

HighQC™ RFP-Human Adipose Derived Mesenchymal Stem Cell

HAdMSCs are isolated from lipoaspirate derived from white adipose tissue and demonstrated with spindle-shaped-, and fibroblast-like cells. RFP-HAdMSCs are puromycin resistant HAdMSCs after trandsfected with lentiviruses expressing RFP under the control of CMV protomer. Each lot of RFP-HAdMSCs is tested to ensure the cells can be passaged at least three times (i.e., approximately 9 to 10 population doublings) after thaw in complete growth media (Mesenchymal Stem Cell Growth Medium (MSCGM)), when cultured following the detailed protocol described below.

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ABC-FC0048

HighQC™ RFP-Human Umbilical Cord-Derived Mesenchymal Stem Cell

HUcMSCs are isolated from umbilical cord matrix (Wharton's Jelly) and demonstrated with spindle-shaped-, and fibroblast-like cells. RFP-HUcMSCs are puromycin resistant HUcMSCs after trandsfected with lentiviruses expressing RFP under the control of CMV protomer. Each lot of RFP-HUcMSCs is tested to ensure the cells can be passaged at least three times (i.e., approximately 9 to 10 population doublings) after thaw in complete growth media (Mesenchymal Stem Cell Growth Medium (MSCGM)), when cultured following the detailed protocol described below.

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ABC-FC0049

GFP-Bovine Aortic Endothelial Cells

GFP-bAECs are selected from bAECs transfected using lentivirus expressing GFP using Puromycin. The cells are shipped in a frozen vial(the cells are provided @ passage 2). Endothelial Growth Medium is recommended for the expansion of GFP-bAECs and these cells can be propagated to at least 12 population doubling times without losing their morphologic and phenotypic characteristics when cultured following the detailed protocol described below.

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ABC-FC0050

RFP-Bovine Aortic Endothelial Cells

RFP-bAECs are selected from bAECs transfected using lentivirus expressing RFP using Puromycin. The cells are shipped in a frozen vial(the cells are provided @ passage 2). Endothelial Growth Medium is recommended for the expansion of RFP-bAECs and these cells can be propagated to at least 12 population doubling times without losing their morphologic and phenotypic characteristics when cultured following the detailed protocol described below.

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ABC-FC0051

GFP-Mouse Brain Microvascular Endothelial Cells

mBMECs were isolated from E18 embryonic brain cortex microvessels of Balb/C mouse and transformed by transfected with SV40 middle T-antigen. GFP-mBMECs were selected puromycin resistant mBMECs from GFP-expressing lentiviral particle. The cells are shipped in frozen vials(the cells are provided @ passage 6) transfected mAECs. The cells are shipped in proliferating culture with 90 confluence (the cells are provided @ passage 4-6). DMEM containing 20% FBS is recommended for cell culture.

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ABC-FC0053

GFP-Mouse Pulmonary Microvascular Endothelial Cells

Mouse Liver Microvascular Endothelial cells (mLivMECs) are isolated from livers of 3-week old male C57BL/6 mice and GFP expressing mLivMECs-GFP were selected from puromycin resistant cells after transfected with lentiviral expressing GFP. Cells are shipped in a frozen vial (provided @ passage 3, with 5 x 105cells/vial). mLivMECs-GFP are cultured with specially formulated Endothelial Growth Medium. Since the cells have limited proliferation capacity (cells may be expanded for a maximum of 2-3 passages) in vitro, long term culture of mLivMECs is not recommended, even cultured following the detailed protocol described below).

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ABC-FC0055

GFP-Mouse Heart Microvascular Endothelial Cells

Mouse Heart Microvascular Endothelial cells (mHtMECs) are isolated from hearts of 3-week old male C57BL/6 mice and GFP expressing mHtMECs-GFP were selected from puromycin resistant cells after transfected with lentiviral expressing GFP. Cells are shipped in a frozen vial (provided @ passage 3, with 5 x 105 cells/vial). mHtMECs-GFP are cultured with specially formulated Endothelial Growth Medium. Since the cells have limited proliferation capacity (cells may be expanded for a maximum of 2-3 passages) in vitro, long term culture of mHtMECs is not recommended, even cultured following the detailed protocol described below.

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ABC-FC0057

GFP-Mouse Liver Microvascular Endothelial Cells

Mouse Liver Microvascular Endothelial cells (mLivMECs) are isolated from livers of 3-week old male C57BL/6 mice and GFP expressing mLivMECs-GFP were selected from puromycin resistant cells after transfected with lentiviral expressing GFP. Cells are shipped in a frozen vial (provided @ passage 3, with 5 x 105 cells/vial). mLivMECs-GFP are cultured with specially formulated Endothelial Growth Medium. Since the cells have limited proliferation capacity (cells may be expanded for a maximum of 2-3 passages) in vitro, long term culture of mLivMECs is not recommended, evencultured following the detailed protocol described below).