Human Subclavian Artery Endothelial Cells (HScAEC ) are derived from normal human subclavian arteries. They are cryopreserved at second passage and can be cultured and propagated at least 10 population doublings.
Monkey Coronary Artery Endothelial Cells from Gentaur are isolated from tissue of Cynomolgus Monkey. Monkey Coronary Artery Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and are delivered frozen.The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of Dynabeads pre-coated with secondary antibody.
Human Subclavian Artery Endothelial Cells (HScAEC ) are derived from normal human subclavian arteries. They are cryopreserved at second passage and can be cultured and propagated at least 10 population doublings.