Human Carotid Artery Endothelial Cells (HCtAEC) are primary endothelial cells isolated from normal carotid arteries and are cryopreserved at third passage. HCtAEC can be propagated at least 10 doublings.
GFP-HAAECs were selected from HAAECs resistant to puromycin after infected with GFP expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 3). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels 14 when cultured following the detailed protocol described below.
Human Carotid Artery Endothelial Cells (HCtAEC) are primary endothelial cells isolated from normal carotid arteries and are cryopreserved at third passage. HCtAEC can be propagated at least 10 doublings.