Ovine Recombinant (E.Coli) Purified Leptin Quadruple mutant mutant Antagonist Protein

rAP-2346

Leptin Antagonist Quadruple Mutant Ovine Recombinant

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LEP26-QM-10

Ovine Recombinant (E.Coli) Purified Leptin Quadruple mutant mutant Antagonist Protein

480.2 €

LEP26-QM-50

Ovine Recombinant (E.Coli) Purified Leptin Quadruple mutant mutant Antagonist Protein

919.4 €

500-067

Ovine Leptin antagonist (mutant L39A/D40A/F41A/I42A) Recombinant Protein

Recombinant ovine leptin, one polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa, ovine leptin was mutated, resulting in L39A/D40A/F41A/I42A mutant was purified by proprietary chromatographic techniques. Preparation of leptin antagonists was published - Niv-Spector et al, Biochem. J 291;221-230 (2005).

449.25 €

500-067S

Ovine Leptin antagonist (mutant L39A/D40A/F41A/I42A) Recombinant Protein

Recombinant ovine leptin, one polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa, ovine leptin was mutated, resulting in L39A/D40A/F41A/I42A mutant was purified by proprietary chromatographic techniques. Preparation of leptin antagonists was published - Niv-Spector et al, Biochem. J 291;221-230 (2005).

394.12 €

500-066

Ovine Leptin pegylated antagonist (mutant L39A/D40A/F41A) Recombinant Protein

Mono-pegylated (with 20 kDa PEG)recombinant ovine leptin antagonist is one polypeptide chain containing 146 amino, an additional Ala at N-terminus and one molecule of PEG 20 kDa at its N-terminus, having an expected molecular mass of ~ 35.6 kDa as determined by MS. However due to enlarged hydrodymanic volume it runs on the SDS-PAGE as 48 kDa protein and in gel-filtration on Superdex 200 as over 200 kDa protein. Ovine leptin was initially mutated, resulting in L39A/D40A/F41A mutant that was purified by proprietary chromatographic techniques according to Niv-Spector et al (2005) Biochemical Journal, 291, 221-230 and its pegylation was similar to that of mouse leptin antagonist is described in Elinav et al. Endocrinology (2009)150, 3083-3091.

701.25 €