Human Carotid Artery Endothelial Cells (HCtAEC) are primary endothelial cells isolated from normal carotid arteries and are cryopreserved at third passage. HCtAEC can be propagated at least 10 doublings.
GFP-HAAECs were selected from HAAECs resistant to puromycin after infected with GFP expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 3). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels 14 when cultured following the detailed protocol described below.