
Katalognummer: 870 - ABC-SC0055T
Produktkategori: Företag och industri > Vetenskap och laboratorium
Storlek: 1 vial
| Storage and shipping | Liquid Nitrogen |
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ABC-SC0055T
Derived from fat tissue of strain Wistar rats (SPF) and cultured as a monolayer for two passages in Adipose-Derived Mesenchymal Stem Cell Growth Medium.Cells are cryopreserved at the second passage.
ABC-SC0057T
Derived from inguinal fat tissue of beagle dogs (SPF) and cultured as a monolayer for two passages in Adipose-Derived Mesenchymal Stem Cell Growth Medium. Cells are cryopreserved at the second passage.
ABC-SC0082
Human Mesenchymal (Adipose Derived) Stem Cell; Frozen Vial and Plated cells are available. Cells are only guaranteed with purchase of Gentaur Media and Gentaur Extra Cellular Matrix for appropriate cell culture, for 30 days from the date of shipment.
ABC-SC0027T
Human MSCs (Bone Marrow Derived) from a single adult donor clinically diagnosed with Type II Diabetes. The MSCs are compatible with our MSC Expansion Medium for Bone Marrow Derived MSCs.All of the cells provided are tested and are negative for HIV-1, HIV-2, Hepatitis B and Hepatitis C as detected by PCR. The cells are confirmed to be negative for mycoplasma and other detectable microbial contamination.
ABC-SC0035T
Mesenchymal Stem Progeitors are derived from human embryonic cell line, H9 (WA09). Cells are andgt;80% CD90, CD105, CD166 and CD73 positive and andlt;5% Oct4 positive. Cells have the capabilties to: Form adherent monolayers – ideal for 96-and 384-well formats for high throughput and high content cell-based assays.To differentiate into a wide variety of mesenchymal subtypes, including osteogenic and chondrogenic lineages (but not adipogenic)– ideal for drug discovery for a wide variety of biological targets and basic research in bone, cartilage, metabolic and immunological diseases.Passage up to 10X.Note: Please include a 30 USD dry ice charge when ordering. Pricing is for US and Canada only.Specifications: Cell Type: mesenchymal progenitor cells Species: human Source: derived from human embryonic cell line, H9 (WA09) Morphology: fibroblasticQuantity: andgt;1x10^6 cells per vial (viable post-thaw) Shipping method: dry iceStorage: liquid nitrogenGrowth Properties: adherent, proliferative Medium: αMEM Medium supplemented with 10% FBS, Pen/Strep and L-glutamine Phenotyping: Cells are andgt;80% CD90, CD105, CD166 and CD73 positive and andlt;5% Oct4 positive. DNA fingerprint: Results are consistent with the presence of a single cell line with no evidence of culture cross contamination. Pathogen testing: This lot was derived from the H9 hESC line that has been tested for Hepatitis B, Hepatitis C, HIV-1, HIV-2, HTLV-I/II, HSV1, HSV2, EBV, and CMV. The H9 cell line has been tested and shown to be negative (performed by GIVF Laboratories). Mesenchymal cells are also tested for sterility and mycoplasma contamination.
ABC-SC0032T
It is well-established that induced pluripotent stem cells may be generated by co-transfection of four genes: Oct3/4, Sox2, Kfl4 and c-Myc, into somatic adult cells resulting in re-programming of these cells to pluripotency equivalent to that of embryonic stem cells. It has recently been shown by independent research including results from our laboratory that over-expression of a single gene, Oct3/4, in adult neural or mesenchymal stem cells also results in enhanced differentiation capacity including pluripotency. This development simplifies iPS generation and may reflect levels of endogenous expression of Sox2, Kfl4 and c-Myc in adult stem cells that are multipotent.Also, since the Oct3/4 promoter has well-known response elements and transcription control mechanisms, re-programming based on environmental cues is now feasible through use of small molecular agents that selectively activate expression of Oct3/4. Our human MSCs expressing Oct3/4 allow further investigation of iPSC mechanisms.Optimizing your hMSC Cultures: 1) Grow cells in 1 to 5% O2, 5% CO2, with the balance of the gas content as N2..2) Use of our MSCGroTM media is highly recommended.3) Subtrate-We recommend Greiner Bio-one TC-treated cell culture flasks or equivalent.

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