HighQC™ Human Neural Stem Cell Line AcceGen

HighQC™ Human Neural Stem Cell Line

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quantity

Produktdetaljer

Katalognummer: 870 - ABC-SC0105T

Produktkategori: Företag och industri > Vetenskap och laboratorium

Leverantör:

AcceGenGentaur

Storlek: 1 vial

Parameters

Storage and shipping	Liquid Nitrogen

ABC-SC0105T

HighQC™ Human Neural Stem Cell Line

Cultures of dissociated NSCs were generated from human fetal brain tissue of 10-14 weeks gestation. NSCs were further cultured under low oxygen conditions and were transduced with retrovirus carrying L-MYC gene.HighlightsGenerated from dissociated NSCs from human fetal brain tissueDisplay both self-renewal and multipotent differentiation into neurons, oligodendrocytes, and astrocytesExpression of the L-MYC gene was confirmed by genomic PCR analysis (Li-Gutova-2016)Found to be non-tumorigenic in vivoFurther characterization shows: a normal, female karyotype, ability to differentiate into neuronal lineages, and demonstrated the lack of abnormal growth or tumorigenicity in vitro or in vivo.Pre-clinical studies indicate that neural stem cells (NSCs) can limit or reverse CNS damage through direct cell replacement, promotion of regeneration, or delivery of therapeutic agents. Immortalized NSC lines are in growing demand due to the inherent limitations of adult patient-derived NSCs, including availability, expandability, potential for genetic modifications, and costs.

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ABC-SC0075T

HighQC™ Human IPSC-Derived Neural Stem Cell

Human Neural Stem Cells from Gentaur are differentiated from a human induced pluripotent stem cell line (hiPSC), which is generated using mRNA reprogramming technology from Human Dermal Fibroblasts (HDF). The monolayer HDF-hiPSC are efficiently converted to neural epithelium using HPSC Neural Induction Medium, a serum-free medium for rapid and efficient neural induction of human pluripotent stem cells (hPSCs). Synergistic inhibition of glycogen synthase kinase 3 (GSK3) and transforming growth factor b (TGF-b) differentiates HDF-hiPSCs to homogenous neural stem cells (NSC) in the presence of human leukemia inhibitory factor (LIF) within 7 days.The derived NSC are characterized by immunofluorescence with antibodies specific to nestin and SOX2. The cell population is highly pure: andgt;90% of cells express Nestin and andgt;80% of cells are SOX2 positive. After reviving, NSC can be maintained in HPSC Neural Induction Medium as an adherent culture or form neural spheres in EGF and bFGF containing neural stem cell medium. The cells are able to form rosette and neural tube-like structures after being passaged and plated at high density on Matrigel coated culture vessels in neural stem medium containing EGF and bFGF. HiPSC-NSC can be further expanded for months as a monolayer on MatrigelTM coated culture vessels or in suspension as neural spheres when seeded on ultra-low binding culture vessels in neural stem cell medium. NSC are multipotent and able to differentiate into various neuronal and glial subtypes. Specific patterning cues, such as SHH, retinoic acid and FGF8, can be added after reviving to direct the cells to different neural lineages.HiPSC-NSC are cryopreserved at passage 0 and delivered frozen. Each vial contains andgt;1 x 10^6 cells in 1 ml volume. Cells are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi.

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ABC-SC0115T

HighQC™ Human Neural Stem Cell: HNSC (Brain Cortex)

Human Neural Stem Cells (HNSC) are self-renewing, generated throughout an adult's life via neurogenesis. These multipotent adult stem cells generate the main phenotype of the nervous system, differentiating into neurons, astrocytes, and oligodendrocytes.HNSC play important roles for development, learning and hippocampal plasticity. They are also used to study age-related declines in proliferation, as well as neurological diseases like stroke, multiple sclerosis, and Parkinson's disease. The cells respond to injury, and can be differentiated to replace lost or injured neurons. They migrate in a directed fashion to brain tumors and help replace dying neurons in injured adult brain tissue.GentaurHNSC are cells derived from the cortex region of human brain (single donor). They are cryopreserved at first passage. Our HNSC stain positive for β-tubulin III, GFAP and oligodendrocyte marker O4 when cultured in Human Neural Differentiation Medium for 10 days. Each lot is tested for its ability to form neurospheres in Human Neural Stem Cell Growth Medium.

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ABC-SC0072

HighQC™ Human Neuronal Stem Cell (Cortex)

Human Neuronal Stem Cells (Cortex); Frozen Vial and Plated cells are available. Cells are only guaranteed with purchase of Gentaur Media and Gentaur Extra Cellular Matrix for appropriate cell culture, for 30 days from the date of shipment.

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ABC-SC0083

HighQC™ Human Melanoma Cancer Stem Cell

Human Melanoma Cancer Stem Cell; Frozen Vial and Plated cells are available. Cells are only guaranteed with purchase of Gentaur Media and Gentaur Extra Cellular Matrix for appropriate cell culture, for 30 days from the date of shipment.

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ABC-SC0088

HighQC™ Human Leukemia Cancer Stem Cell

Human Leukemia Cancer Stem Cell; Frozen Vial and Plated cells are available. Cells are only guaranteed with purchase of Gentaur Media and Gentaur Extra Cellular Matrix for appropriate cell culture, for 30 days from the date of shipment.

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