Katalognummer: 763 - E-EL-R0269
Produktkategori: Företag och industri > Vetenskap och laboratorium
Storlek: 1 plate of 96 wells
Additional information | Gentaur's CORT ELISA kit utilizes the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat CORT. During the reaction, Rat CORT in the sample or standard competes with a fixed amount of Rat CORT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat CORT. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat CORT in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Storage and shipping | The kit is shipped on ice packs or blue ice. Upon receving it, keep refrigerated at 1 to 8 degress Celsius. The shelf life of this is guaranteed to be at least 6 months from the date of receipt. |
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