
Katalognummer: 855 - ELK8156
Produktkategori: Företag och industri > Vetenskap och laboratorium
Storlek: 1 plate of 96 wells
| Additional information | A monoclonal antibody specific to 12-Hydroxyeicosatetraenoic Acid (12-HETE) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 12-Hydroxyeicosatetraenoic Acid (12-HETE) and unlabeled 12-Hydroxyeicosatetraenoic Acid (12-HETE) (Standards or samples) with the pre-coated antibody specific to 12-Hydroxyeicosatetraenoic Acid (12-HETE). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 12-Hydroxyeicosatetraenoic Acid (12-HETE) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 12-Hydroxyeicosatetraenoic Acid (12-HETE) in the sample. |
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| Storage and shipping | The General 12-HETE ELISA kit is shipped on ice packs. Upon receipt keep refrigerated at + 4 °C. |

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