ELISA kit for 12-HETE (12-Hydroxyeicosatetraenoic Acid)

ELISA kit for 12-HETE (12-Hydroxyeicosatetraenoic Acid)

41054 EUR

41054 EUR
In Stock
quantity
Produktdetaljer

Katalognummer: 763 - E-EL-0109

Produktkategori: Företag och industri > Vetenskap och laboratorium

Leverantör:

Elabscience BiotechGentaur

Storlek: 1 plate of 96 wells

Parameters

Additional informationGentaur's 12-HETE ELISA kit utilizes the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 12-HETE. During the reaction, 12-HETE in the sample or standard competes with a fixed amount of 12-HETE on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 12-HETE. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 12-HETE in the samples is then determined by comparing the OD of the samples to the standard curve.
Storage and shippingThe kit is shipped on ice packs or blue ice. Upon receving it, keep refrigerated at 1 to 8 degress Celsius. The shelf life of this is guaranteed to be at least 6 months from the date of receipt.