Rat Intestinal Epithelial Cells
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Produktdetaljer
Katalognummer: 870 - ABC-TC4140
Produktkategori: Företag och industri > Vetenskap och laboratorium
Gentaur
Storlek: 1 vial
ABC-TC3879
Human Intestinal Epithelial Cells
Cryopreserved InEpC are truly primary cells representing both villi (enterocytes, goblet, and enteroendocrine cells) and crypts structures. In combination with human intestinal myofibroblasts (InMyoFib).
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ABC-TC4140
Rat Intestinal Epithelial Cells
Rat Intestinal Epithelial Cells from Gentaur are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats. Rat Intestinal Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Intestinal Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin and ZO-1. Rat Intestinal Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.
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ABC-TC5510
Porcine Small Intestinal Epithelial Cells
Porcine Primary Small Intestinal Epithelial Cells from Gentaur are isolated from small intestinal tissue of porcine. Porcine Primary Small Intestinal Epithelial Cells are grown in a T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Gentaur's Culture Complete Growth Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x106 cells per ml and is delivered frozen. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with EpCAM-1 (CD326) antibody, following the application of magnetic beads pre-coated with secondary antibody.
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ABC-TC3669
Human Lens Epithelial Cells
Human Lens Epithelial Cells from Gentaur Research Laboratories are isolated from the human lens. HLEpiC are cryopreserved at primary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HLEpiC are characterized by immunofluorescent method with antibodies to cytokeratin-18, cytokeratin-19 and fibronectin. HLEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HLEpiC are guaranteed to further culture in the conditions provided by Gentaur Research Laboratories.
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ABC-TC3719
Human Nasal Epithelial Cells
The cells are isolated from normal human adult tissues of the airways (nasal mucosa, trachea, bronchi, and distal respiratory tract), breast (mammary gland), kidney (whole kidney and renal cortex), and the placenta (amniotic membrane). Shortly after isolation, all Gentaur Human Epithelial Cells are cryopreserved at passage 2 (P2) . Each cryo vial contains more than 500,000 viable cells after thawing. Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at Gentaur.
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ABC-TC3744
Human Pancreatic Epithelial Cells
Human Pancreatic Epithelial Cell were initiated by elutriation of dispase dissociated normal pancreatic tissue.These cells were originated using Complete Serum-Free Medium Kit With AcceSup™, are available at <12 Cumulative Population Doublings (CPD) in vitro [Passage 3] and were cryopreserved in aliquots of ~1.5×10^6 cells. This vial will initiate a Passage 4 cell culture in a 75cm2 flask.