PDE7A Assay Kit

60370

PDE7A Assay Kit

The PDE7A Assay Kit is designed_x000D_for identification of PDE7A inhibitors using fluorescence polarization. The assay is_x000D_based on the binding of a fluorescent nucleotide monophosphate generated by PDE7A_x000D_to the binding agent. The key to the PDE7A_x000D_Assay Kit is the specific binding agent. Using this kit, only two simple steps on a_x000D_microtiter plate are required for PDE7A reactions. First, the fluorescently labeled cAMP_x000D_is incubated with a sample containing PDE7A for 1 hour. Second, a binding agent is_x000D_added to the reaction mix to produce a change in fluorescent polarization that can then_x000D_be measured using a fluorescence reader.

757.5 €

60373

PDE7A Assay Kit

The PDE7A Assay Kit is designed for identification of PDE7A inhibitors using_x000D_fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide_x000D_monophosphate generated by PDE7A to the binding agent. PDE7A catalyzes the hydrolysis of_x000D_the phosphodiester bond in dye-labeled cyclic adenosine monophosphate (cAMP)._x000D_Nanoparticle beads selectively bind the phosphate group in the nucleotide product. This_x000D_increases the size of the nucleotide relative to unreacted cAMP. Since the degree of polarization_x000D_of a fluorophore is inversely related to its molecular rotation, dyes attached to the slowly-rotating_x000D_nucleotide-bead complexes will not have time to reorient and therefore will emit highly polarized_x000D_light. Conversely, dyes attached to the rapidly-rotating cyclic monophosphates will obtain_x000D_random orientations and emit light with low polarization. The key to the PDE7A Assay Kit is the specific_x000D_binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE7A_x000D_reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE7A_x000D_for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in_x000D_fluorescent polarization that can then be measured using a fluorescence reader.

1350 €

GWB-PS0CBC

PDE7A Assay Kit

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