MCF7 Lysate
440 EUR
In Stock
quantity
Produktdetaljer
Katalognummer: 544 - MBS151733-01mg
Produktkategori: Företag och industri > Vetenskap och laboratorium
Gentaur
Storlek: 0.1mg
Parameters
| Storage and shipping | Store at 2-8 degree C for continuous use. For extended storage, freeze working aliquots at -70 degree C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt. |
|---|
1219
MCF7 Lysate
MCF7 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The MCF7 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.
473.8 €
SC050-G
MCF7 / GFP (Puromycin) Stable Cell Line
GFP expression stable cell line in MCF7 cells with Puromycin resistance
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