Ion Selective Electrode Std Nitrate 100ppm as NO3 - 5L

ISEN1005-5L

Ion Selective Electrode Std Nitrate 100ppm as NO3 - 5L

1188.05 €

ISEN1005

Ion Selective Electrode Std Nitrate 100ppm as NO3 - 500ML

398.17 €

ISEN105-5L

Ion Selective Electrode Std Nitrate 10ppm as NO3 - 5L

1168.21 €

ISEPCL5

Ion Selective Electrode Std Perchlorate 1000ppm - 500ML

324.39 €

ISESC5

Ion Selective Electrode Std Thiocyanate 1000ppm - 500ML

339.45 €

3330-10

ION MDR Activity Kit

A Calcein AM efflux assay to screen a wide range of compounds for multidrug resistance transporter activityExcitation: 528 nm | Emission: 617 nmCalcein AM is a membrane-permeant, non-fluorescent dye that enters cells passively. Once inside the cytosol of cells, intracellular esterases convert it to fluorescent Calcein (Ex/Em: 495 nm/515 nm), resulting in uniform cytosolic fluorescence. Drug efflux transporters, such as P-glycoprotein (Pgp, MDR1) and multidrug-resistance-associated protein (MRP1), actively extrude Calcein AM from inside the cell before esterases can convert it to Calcein. The presence of additional MDR substrates or inhibitors of MDR expression results in decreased Calcein AM efflux, causing a measurable increase in intracellular fluorescence. In addition to identifying MDR substrates and inhibitors, this kit can also be used to evaluate the activity of MDR transporters in cells.When following our protocol, ION’s MDR Activity kit provides enough reagents to make 100 mL of working solution, enough for ten 96- or 384-well plates or 80 flow cytometry samples. The actual number of assays will vary according to optimal dye concentrations for your application.

257.55 €