ION MDR Activity Kit ION Biosciences

ION MDR Activity Kit

257.55 EUR

In Stock

quantity

Produktdetaljer

Katalognummer: 1140 - 3330-10

Produktkategori: Företag och industri > Vetenskap och laboratorium

Leverantör:

ION BiosciencesGentaur

Storlek: 1 kit

Parameters

Storage and shipping	Insulated Cooler, Overnight

5000

ION Vital - Viability Kit, live or dead assay kit for mammalian cells

A rapid and simple two-color assay to discriminate between live and dead cells. Compatible with fluorescence microscopy, flow cytometry, and plate reader assays.Live: Ex 495 nm / Em 515 nm | Dead: Ex 528 nm / Em 617 nmION Vital - Viability kit can be used to quantify live and dead cells within eukaryotic cell suspensions or adherent cultures, 3D cultures, organoids, and some non-fixed tissues. Cannot be used for yeast or bacteria. When following our protocol, the Viability kit provides enough reagents to make 150 mL of working solution, enough for fifteen 96-well plates or 1,500 flow cytometry samples. The actual number of assays will vary according to optimal dye concentrations for your application.Calcein AM is a membrane-permeant, non-fluorescent form of calcein that enters cells passively. Once inside the cytosol of cells, Calcein AM is converted to green fluorescent Calcein by ubiquitous esterases in viable cells, resulting in uniform cytosolic fluorescence (Ex/Em 495 nm/515 nm). Calcein, a polyanionic dye, is membrane-impermeant and is well retained within the cytosol of most healthy cells with intact cell membranes. Ethidium homodimer I (EthD-I) is a membrane-impermeable, high-affinity, nucleic acid stain that is excluded by viable cells with intact cell membranes. When membrane integrity is compromised, a hallmark of dead or dying cells, EthD-I enters the cell and binds DNA, which results in a >30-fold enhancement in bright red, nuclear fluorescence (Ex/Em 528 nm/617 nm).

205.7 €

1497527

ION Essentials Ratiometric Calcium - No wash ratiometric calcium assay kit

No wash, ratiometric calcium flux assay, 2 plates. Total assay solution compatible with plate reader and fluorescence microscopy applications. Excitation: 340, 380 nm | Emission: 505 nm | Kd: 145 nMRatiometric Calcium Essentials kit provides the necessary reagents for conducting no wash, ratiometric calcium flux assays compatible with plate reader and fluorescence microscopy applications. Individual components are provided to give the user the flexibility needed to customize your assay.

144.5 €

6000-10

ION Essentials Ratiometric Calcium - No wash ratiometric calcium assay kit

No wash, ratiometric calcium flux assay, 10 plates. Total assay solution compatible with plate reader and fluorescence microscopy applications. Excitation: 340, 380 nm | Emission: 505 nm | Kd: 145 nMRatiometric Calcium Essentials kit provides the necessary reagents for conducting no wash, ratiometric calcium flux assays compatible with plate reader and fluorescence microscopy applications. Individual components are provided to give the user the flexibility needed to customize your assay.

317.9 €

1041G

Fluo-4 AM - Green fluorescent calcium indicator

Calcium indicator, membrane permeable. Compatible with flow cytometry, plate reader, and fluorescence microscopy assays.Excitation: 490 nm | Emission: 515 nm | Kd: 355 nM | Purity: >95%Fluo-4 is the most used and the most trusted of the single-wavelength Ca²⁺ indicators. It has been the indicator of choice for countless basic research studies and has been the used to screen many tens-of-millions of compounds using high-throughput screening (HTS) to discover pharmacological tools and drugs for GPCRs, and a wide variety of Ca²⁺ permeable ion channels and transporters.

57.8 €

1042C

Fluo-4 K+ Salt - Green fluorescent calcium indicator

Calcium indicator, membrane impermeable. Compatible with flow cytometry, plate reader, and fluorescence microscopy assays.Excitation: 490 nm | Emission: 515 nm | Kd: 355 nM | Purity: >95%Fluo-4 is the most used and the most trusted of the single-wavelength Ca²⁺ indicators. It has been the indicator of choice for countless basic research studies and has been the used to screen many tens-of-millions of compounds using high-throughput screening (HTS) to discover pharmacological tools and drugs for GPCRs, and a wide variety of Ca²⁺ permeable ion channels and transporters.Fluo-4 K⁺ Salt is a membrane impermeable form of Fluo-4 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.

124.95 €

1042F

Fluo-4 K+ Salt - Green fluorescent calcium indicator

124.95 €

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