First, fetal liver is homogenized. Then, a density separation gradient media (1.077g/ml density) is used to isolate the Mononuclear Cells. Next, a direct immunomagnetic CD34 MicroBead labeling system is used to positively isolate FL-CD34+ cells.
Fetal liver is homogenized, and then a density separation gradient media (1.077 g/ml density) is used to isolate the mononuclear cells. Next, a direct immunomagnetic CD133 MicroBead labeling system is used to positively select FL-CD133+ cells. The majority of CD133+ cells express the CD34 cell marker.
First, fetal liver is homogenized. Then, a density separation gradient media (1.077g/ml density) is used to isolate the Mononuclear Cells. Next, a direct immunomagnetic CD34 MicroBead labeling system is used to positively isolate FL-CD34+ cells.