HeLa Lysate
1307 EUR
In Stock
quantity
Produktdetaljer
Katalognummer: 544 - MBS151603-5x01mg
Produktkategori: Företag och industri > Vetenskap och laboratorium
Gentaur
Storlek: 5x0.1mg
Parameters
| Storage and shipping | Store at 2-8 degree C for continuous use. For extended storage, freeze working aliquots at -70 degree C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt. |
|---|
1201
HeLa Lysate
HeLa lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The HeLa lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.
473.8 €
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