HDAC1 Fluorogenic Assay Kit

50061

HDAC1 Fluorogenic Assay Kit

The Fluorogenic HDAC1 Assay Kit is a complete assay system_x000D_designed to measure histone deacetylase 1 (HDAC1) activity for screening and profiling_x000D_applications. It comes in a convenient 96-well format, with all the reagents necessary for_x000D_100 fluorescent HDAC1 activity measurements. In addition, the kit includes purified_x000D_HDAC1 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and_x000D_negative control. The Fluorogenic HDAC1 Assay Kit is based on a unique fluorogenic_x000D_substrate and developer combination. This assay method eliminates dealing with the_x000D_radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit,_x000D_only two simple steps on a microtiter plate are needed to analyze the HDAC activity_x000D_level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain,_x000D_is incubated with purified HDAC1. The deacetylation sensitizes the substrate so_x000D_subsequent treatment with the Lysine Developer produces a fluorophore that can then_x000D_be measured using a fluorescence reader._x000D_

847.5 €

GWB-PSF8DD

HDAC1 Fluorogenic Assay Kit

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50687

HDAC11 Fluorogenic Assay Kit

The HDAC11 Fluorogenic Assay Kit is a complete assay system designed to measure histone deacetylase 11 (HDAC11) activity for screening and profiling applications. The HDAC11 Fluorogenic Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the HDAC11 activity level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain, is incubated with purified HDAC11. The deacetylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader._x000D_HDACs regulate cellular processes by catalyzing the hydrolysis of an acetyl group from acetyllysines in modified proteins. In the HDAC assay, fluorescent-dye molecules are attached to a peptide containing acetyllysine. Attachment to the peptide quenches the fluorescence of the dye. After treatment of the peptide with an HDAC, the reaction is mixed with a development solution that is specific for nonacetylated lysines. If the acetyl group has been removed from the lysine by the HDAC, this solution will release the dye allowing for fluorescence. The amount of fluorescence is therefore directly related to the level of HDAC activity._x000D_

847.5 €

CB5326

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HDAC8 Cell-Based ELISA Kit

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HDAC9 Cell-Based ELISA Kit

HDAC9 Colorimetric Cell-Based ELISA Kit

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