EZH2 (Y641F) Chemiluminescent Assay Kit

52075

EZH2 (Y641F) Chemiluminescent Assay Kit

The EZH2 (Y641F) Chemiluminescent Assay Kit is designed to_x000D_measure activity of the mutant EZH2 complex (EZH2 (Y641F)/EED/SUZ12/RbAp48/_x000D_AEBP) for screening and profiling purposes. The key to the_x000D_EZH2 (Y641F) Chemiluminescent Assay Kit is a highly specific antibody that recognizes_x000D_methylated Histone H3K27. With this kit, only three simple steps on a microtiter plate are_x000D_required for methyltransferase detection. First, S-adenosylmethionine is incubated with a_x000D_sample containing assay buffer and methyltransferase enzyme for one hour. Next,_x000D_primary antibody is added. Finally, the strips are treated with an HRP-labeled secondary_x000D_antibody followed by addition of the HRP substrate to produce chemiluminescence that_x000D_can then be measured using a chemiluminescence reader.

2475 €

52680

EZH2(Y641F) TR-FRET Assay Kit

The EZH2 (Y641F) TR-FRET Assay Kit is designed to measure activity of the mutant EZH2 complex (EZH2 (Y641F)/EED/SUZ12/RbAp48/ AEBP) in a homogeneous 384 reaction format. This FRET-based assay requires no timeconsuming washing steps, making it especially suitable for high throughput screening applications. The EZH2 (Y641F) TR-FRET Assay Kit comes in a convenient format, with histone H3 peptide substrate, a Tb-labeled antibody against methylated K27 residue of Histone H3, S-adenosylmethionine, methyltransferase assay buffer, TR-FRET detection buffer, dye-labeled acceptor, and purified EZH2 (Y641F) complex for 384 enzyme reactions. The key to the EZH2 (Y641F) Assay Kit is a highly specific antibody that recognizes methylated Histone H3K27. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for 240 minutes. Next, antibody is added. Finally, dye-labeled acceptor is added followed by fluorescence reading.

2475 €

79277

EZH2 (Y641N) Chemiluminescent Assay Kit

The EZH2 (Y641N) Chemiluminescent Assay Kit is designed to measure activity of the mutant EZH2 complex (EZH2 (Y641N)/EED/SUZ12/RbAp48/ AEBP) for screening and profiling purposes.

4275 €

MBS8533475-01mL

EZH2

624.88 €

MBS8533475-01mLAF405L

EZH2

905.38 €

MBS8533475-01mLAF405S

EZH2

905.38 €