ELISA kit for Mouse C3a (Complement Component 3a)

ELISA kit for Mouse C3a (Complement Component 3a)

58010 EUR

58010 EUR
In Stock
quantity
Produktdetaljer

Katalognummer: 763 - E-EL-M0337

Produktkategori: Företag och industri > Vetenskap och laboratorium

Leverantör:

Elabscience BiotechGentaur

Storlek: 1 plate of 96 wells

Parameters

Additional informationGentaur's C3a ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse C3a. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse C3a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse C3a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse C3a. You can calculate the concentration of Mouse C3a in the samples by comparing the OD of the samples to the standard curve.
Storage and shippingThe kit is shipped on ice packs or blue ice. Upon receving it, keep refrigerated at 1 to 8 degress Celsius. The shelf life of this is guaranteed to be at least 6 months from the date of receipt.