CF1 Mouse Embryonic Fibroblasts
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Catalog number: 870 - ABC-TC3333
Product Category: Business & Industrial > Science & Laboratory
Gentaur
Size: 1 vial
ABC-TC3333
CF1 Mouse Embryonic Fibroblasts
CF1 Mouse Primary Embryonic Fibroblasts from Gentaur are isolated from tissue of pathogen-free laboratory mice. Mouse Primary Embryonic Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 2x10^6 cells per ml and is delivered frozen.Mouse Primary Embryonic Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining. Cells can be expanded for 3-6 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.
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ABC-TC4128
Rat Embryonic Fibroblasts
Rat primary embryonic fibroblasts are isolated from day 13.5 embryos of Sprague-Dawley rats. Rat primary embryonic fibroblasts are grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 1 hour and incubated in Culture Complete Growth Medium.
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ABC-TC1343
Mouse Pancreatic Carcinoma Associated Fibroblasts
Mouse Carcinoma Associated Fibroblasts are isolated from human xenograft of pathogen-free laboratory nude mice. Human pancreatic adenocarcinoma cells (BX-PC-3) were subcutaneously injected into the nude mice for 4-6 weeks and the tumor tissues were then harvested for cell culture.
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ABC-TC1344
Mouse Breast Carcinoma Associated Fibroblasts
Mouse Carcinoma Associated Fibroblasts are isolated from human xenograft of pathogen-free laboratory nude mice. Human breast adenocarcinoma cells (MDA-MB-231) were subcutaneously injected into the nude mice for 4-6 weeks and the tumor tissues were then harvested for cell culture.