Cas9-Expressing HCT116 Cell line – High expression
9900 EUR
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Produktdetaljer
Katalognummer: 421 - 78135-H
Produktkategori: Företag och industri > Vetenskap och laboratorium
Gentaur
Storlek: 2 vials
78135-H
Cas9-Expressing HCT116 Cell line – High expression
This cell line is a clonal derivative from the Cas9-Expressing HCT116 Cell Pool (BPS Bioscience, #78073). It was generated by limited dilution of the original pool and isolation of individual clones, which were screened based on Cas9 expression to obtain a high-expressing cell line. The expressed Cas9 protein includes a C-terminal FLAG tag.
9900 €
78135-L
Cas9-Expressing HCT116 Cell line – Low expression
This cell line is a clonal derivative from the Cas9-Expressing HCT116 Cell Pool (BPS Bioscience, #78073). It was generated by limited dilution of the original pool and isolation of individual clones, which were screened based on Cas9 expression to obtain a low-expressing cell line. The expressed Cas9 protein includes a C-terminal FLAG tag.
9900 €
78073
Cas9-Expressing HCT116 cell pool
Cas9 (Streptococcus pyogenes CRISPR associated protein 9) is an endonuclease enzyme that, when recruited to a specific DNA sequence by the sgRNA (single guide RNA), introduces a double stranded break into the DNA. This double stranded break is repaired in mammalian cells either through Non-Homologous End Joining or Homologous Recombination. Non-Homologous End Joining often results in the deletion or insertion of several base pairs at the cut site, which, when resulting in a frameshift, causes the functional inactivation of the targeted gene. Cas9-expressing HCT116 cells can be transduced or electroporated with sgRNA targeting a gene of interest to quickly generate knock-out cell pools or cell lines.
1342.5 €
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