C57BL/6 Mouse Artery Endothelial Cells
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Catalog number: 870 - ABC-TC3165
Product Category: Business & Industrial > Science & Laboratory
Gentaur
Size: 1 vial
ABC-TC4027
Porcine Artery Endothelial Cells
Porcine Artery Endothelial Cells are derived from healthly porcinine artery. Porcine Artery Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and are delivered frozen.Porcine Artery Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation, or transendothelial resistance (TER). Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.
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ABC-TC5472
BKS DB Mouse Artery Endothelial Cells
BKS db Mouse Artery Endothelial Cells are isolated from the artery of Mice homozygous for the diabetes spontaneous mutation (Lepr/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoRIemic. BKS db Mouse Artery Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in Gentaur's Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vials. Each vial contains at least 0.5×106 cells per ml. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic beads pre-coated with secondary antibody.
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ABC-TC3165
C57BL/6 Mouse Artery Endothelial Cells
C57BL/6 Mouse Artery Endothelial Cells from Gentaur are isolated from abdominal aorta tissue of pathogen-free laboratory mice. Mouse Artery Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and are delivered frozen.Mouse Artery Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation, or transendothelial resistance (TER). Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.
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ABC-FC0011
GFP-Human Coronary Artery Endothelial Cells
GFP-HCAECs were selected from HCAECs resistant to puromycin after infected with GFP expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 5). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels 14 when cultured following the detailed protocol described below.
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ABC-FC0021
GFP-Human Umbilical Artery Endothelial Cells
HUAECs are pooled cells isolated from normal human umbilical artery. GFP-HUAECs were selected from HUAECs infected with GFP-expressing lentiviruse with Puromycin and the cells are shipped in frozen vials (the cells are provided @ passage 3). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels 20 when cultured following the detailed protocol.
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ABC-FC0022
RFP-Human Umbilical Artery Endothelial Cells
HUAECs are pooled cells isolated from normal human umbilical artery. RFP-HUAECs were selected from HUAECs infected with RFP-expressing lentiviruses with Zyocin and the cells are shipped in frozen vials (the cells are provided @ passage 3). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels 20 when cultured following the detailed protocol.