C57BL/10J Mouse Heart Fibroblasts
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Catalog number: 870 - ABC-TC3148
Product Category: Business & Industrial > Science & Laboratory
Gentaur
Size: 1 vial
ABC-TC3072
BALB/c Mouse Heart Fibroblasts
Mouse primary cardiac fibroblasts are isolated from tissue of pathogen-free laboratory mice. Mouse primary cardiac fibroblasts are grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 1 hour and incubated in Culture Complete Growth Medium generally for 3-7 days.
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ABC-TC3148
C57BL/10J Mouse Heart Fibroblasts
Mouse primary cardiac fibroblast are isolated from tissue of pathogen-free laboratory mice. Mouse primary cardiac fibroblasts are grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 1 hour and incubated in Culture Complete Growth Medium generally for 3-7 days.
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ABC-TC3091
BALB/c Mouse Mammary Fibroblasts
BALB/c Mouse Primary Mammary Fibroblasts from Gentaur are isolated from tissue of pathogen-free laboratory mice. Mouse Primary Mammary Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Mouse Primary Mammary Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.
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ABC-TC3095
BALB/c Mouse Ovarian Fibroblasts
BALB/c Mouse Primary Ovarian Fibroblasts from Gentaur are isolated from tissue of pathogen-free laboratory mice. Mouse Primary Ovarian Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Mouse Primary Ovarian Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.
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ABC-TC3104
BALB/c Mouse Spleen Fibroblasts
Mouse spleen fibroblasts are isolated from tissue of pathogen-free laboratory mice. Mouse spleen fibroblasts are grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 1 hour and incubated in Culture Complete Growth Medium generally for 3-7 days.