ABC-TC4222

Rat Spleen Fibroblasts

Rat Primary Spleen Fibroblasts from Gentaur are isolated from tissue of neonatal Sprague-Dawley Rats. Rat Primary Spleen Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Spleen Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 2-4 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

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CSC-C4868L

Human Spleen Fibroblasts

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ABC-TC3104

BALB/c Mouse Spleen Fibroblasts

Mouse spleen fibroblasts are isolated from tissue of pathogen-free laboratory mice. Mouse spleen fibroblasts are grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 1 hour and incubated in Culture Complete Growth Medium generally for 3-7 days.

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ABC-TC3070

BALB/c Mouse Embryonic Fibroblasts

Mouse Embryonic Fibroblasts from are isolated from day 13 mouse embryos of pathogen-free laboratory mice. They are grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 1 hour and incubated in Culture Complete Growth Medium for 3-7 days.

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ABC-TC3072

BALB/c Mouse Heart Fibroblasts

Mouse primary cardiac fibroblasts are isolated from tissue of pathogen-free laboratory mice. Mouse primary cardiac fibroblasts are grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 1 hour and incubated in Culture Complete Growth Medium generally for 3-7 days.

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ABC-TC3079

BALB/c Mouse Kidney Fibroblasts

Mouse kidney fibroblasts are isolated from tissue of pathogen-free laboratory mice. Mouse kidney fibroblasts are grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 1 hour and incubated in Culture Complete Growth Medium generally for 3-7 days.

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