ARE Report Cell Line (Nrf2 antioxidant pathway)
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Catalog number: 870 - ABC-RC120D
Product Category: Business & Industrial > Science & Laboratory
Gentaur
Size: 1 vial
ABC-RC120D
ARE Report Cell Line (Nrf2 antioxidant pathway)
The ARE Reporter andndash; Hep G2 cell line is designed to monitor Nrf2 antioxidant response pathway. The cell contains a firefly luciferase gene under the control of ARE (antioxidant response element) stably integrated into Hep G2 cells.
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60513
ARE Reporter – HepG2 Cell line (Nrf2 Antioxidant Pathway)
The ARE Reporter - Hep G2 cell line is designed to monitor Nrf2 antioxidant response pathway. The cell contains a firefly luciferase gene under the control of ARE (antioxidant response element) stably integrated into Hep G2 cells.
3855.68 €
ABC-RC001D
Nrf2 Luciferase Report Cell Line-MCF7
The Nrf2 Luciferase Reporter cell line is a stably transfected MCF7 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the antioxidant response element (ARE). ARE is known to regulate expression and induction of various detoxifying enzyme genes in response to antioxidants and xenobiotics, and is primarily regulated by the Keap1-Nrf2 pathway in which induction and nuclear translocation of Nrf2 mediated by antioxidants and xenobiotics results in the binding of Nrf2 to ARE leading to the expression of defensive genes.
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ABC-RC127D
loxP-GFP-RFP(Bsd) Report Cell Line-HEK293
The CRE reporting cell lines are used to monitor or confirm the efficiency of CRE recombination in vivo. It is a great method and easy tool to verify the performance of your CRE enzyme(your CRE expression plasmids, or pre-made CRE expression lentivirus, or purified CRE enzyme) in vivo conditions. It is also a control test to verify your CRE-loxP based system. Transformed from HEK293 cells, expressing "LoxP-GFP-stop-LoxP-RFP" cassette under CMV promoter(see Color-Switch cassette below). Cell line also contains a blasticidin antibiotic marker under RSV promoter.The cell line demonstrates strong GFP fluorescent signal in normal culture condition as the constitutive CMV promoter drives the GFP high expression to the stop codon. The downstream RFP ORF was not expressed because of the stop codon after the GFP ORF. Once the CRE protein was present in nuclear, the CRE excises/deletes the DNA fragment between two loxP sites, which removes the stop codon(see the DNA structure scheme above). As a result, the RFP ORF is then expressed under the CMV promoter, and the cell line switches to RFP fluorescent. The RFP signal can be easily monitored via fluorescent cell sorting(for the ratio between GFP and RFP cells), or visualized under microscope, or measured the fluorescent intensity by a meter or reader with RFP filter set.
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ABC-RC128D
loxP-GFP-RFP(Neo) Report Cell Line-HEK293
The CRE reporting cell lines are used to monitor or confirm the efficiency of CRE recombination in vivo. It is a great method and easy tool to verify the performance of your CRE enzyme(your CRE expression plasmids, or pre-made CRE expression lentivirus, or purified CRE enzyme) in vivo conditions. It is also a control test to verify your CRE-loxP based system. Transformed from HEK293 cells, expressing "LoxP-GFP-stop-LoxP-RFP" cassette under CMV promoter(see Color-Switch cassette below). Cell line also contains a Neomycin antibiotic marker under RSV promoter.The cell line demonstrates strong GFP fluorescent signal in normal culture condition as the constitutive CMV promoter drives the GFP high expression to the stop codon. The downstream RFP ORF was not expressed because of the stop codon after the GFP ORF. Once the CRE protein was present in nuclear, the CRE excises/deletes the DNA fragment between two loxP sites, which removes the stop codon(see the DNA structure scheme above). As a result, the RFP ORF is then expressed under the CMV promoter, and the cell line switches to RFP fluorescent. The RFP signal can be easily monitored via fluorescent cell sorting(for the ratio between GFP and RFP cells), or visualized under microscope, or measured the fluorescent intensity by a meter or reader with RFP filter set.
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ABC-RC129D
loxP-GFP-RFP(Puro) Report Cell Line-HEK293
The CRE reporting cell lines are used to monitor or confirm the efficiency of CRE recombination in vivo. It is a great method and easy tool to verify the performance of your CRE enzyme(your CRE expression plasmids, or pre-made CRE expression lentivirus, or purified CRE enzyme) in vivo conditions. It is also a control test to verify your CRE-loxP based system. Transformed from HEK293 cells, expressing "LoxP-GFP-stop-LoxP-RFP" cassette under CMV promoter(see Color-Switch cassette below). Cell line also contains a Puromycin antibiotic marker under RSV promoter.The cell line demonstrates strong GFP fluorescent signal in normal culture condition as the constitutive CMV promoter drives the GFP high expression to the stop codon. The downstream RFP ORF was not expressed because of the stop codon after the GFP ORF. Once the CRE protein was present in nuclear, the CRE excises/deletes the DNA fragment between two loxP sites, which removes the stop codon(see the DNA structure scheme above). As a result, the RFP ORF is then expressed under the CMV promoter, and the cell line switches to RFP fluorescent. The RFP signal can be easily monitored via fluorescent cell sorting(for the ratio between GFP and RFP cells), or visualized under microscope, or measured the fluorescent intensity by a meter or reader with RFP filter set.