80569
The PARP1 Chemiluminescent Activity Assay Kit is designed to measure_x000D_PARP1 activity for screening and profiling applications. PARP1 is known to catalyze the NADdependent_x000D_addition of poly (ADP-ribose) to histones. The key to the PARP1 Chemiluminescent_x000D_Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for_x000D_PARP1 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP1_x000D_biotinylated substrate is incubated with an assay buffer that contains the PARP1 enzyme._x000D_Finally, the plate is treated with streptavidin-HRP followed by addition of the HRP substrate to_x000D_produce chemiluminescence that can then be measured using a chemiluminescence reader.
2115 €