TAF1 (BD2) TR-FRET Assay Kit BPS Bioscience

TAF1 (BD2) TR-FRET Assay Kit

1687.5 EUR

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Produktdetaljer

Katalognummer: 421 - 32627

Produktkategori: Företag och industri > Vetenskap och laboratorium

Leverantör:

BPS BioscienceGentaur

Storlek: 384 rxns.

32628

TAF1 (BD1+BD2) TR-FRET Assay Kit

The TAF1 (BD1+BD2) TR-FRET Assay Kit is designed to measure the inhibition of TAF1 (BD1+BD2) binding to its substrate in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing trFluor™ europium (Eu)-labeled TAF1 (BD1+BD2), dye-labeled acceptor, substrate, and an inhibitor is incubated for 120 minutes. Then, the fluorescence intensity is measured using a fluorescence reader.

915 €

32624

TAF1 (BD2) Inhibitor Screening Assay Kit

The TAF1 (BD2) Inhibitor Screening Assay Kit is designed to measure the inhibition of TAF1 (BD2) binding to its substrate. The key to the TAF1 (BD2) Inhibitor Screening Assay Kit is the specific binding of the TAF1 bromodomain 2 (a.a. 1519-1651) to the acetylated histone substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing TAF1 (BD2) and an inhibitor of choice is incubated with the biotinylated substrate for thirty minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.

1687.5 €

32604

TAF1 (BD1/BD2) Inhibitor Screening Assay Kit

The TAF1 (BD 1 + BD2) Inhibitor Screening Assay Kit is designed to measure the inhibition of TAF1 binding to its substrate. The key to the kit is the specific binding of the tandem TAF1 bromodomains to the acetylated histone substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing TAF1 and an inhibitor of choice is incubated with the biotinylated substrate for thirty minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts

1687.5 €

78306

XIAP intrachain TR-FRET Assay Kit

Covalent conjugation to ubiquitin (Ub) is one of the major post-translational modifications that regulates protein stability, function, and localization. Ubiquitination is the concerted action of three enzymes: a Ub-activating enzyme (E1), a Ub-conjugating enzyme (E2), and a Ub ligase (E3). The specificity and efficiency of ubiquitination are largely determined by the E3 enzyme, which directs the last step of the Ub-conjugating cascade by binding to both an E2~Ub conjugate and a substrate protein. This step ensures the transfer of Ub from E2~Ub to the substrate, leading to its mono- or poly-ubiquitination.The X-linked inhibitor of apoptosis (XIAP) protein is a RING-containing E3 Ub ligase which has the ability to directly regulate caspases and suppress apoptotic cell death pathways. An increased expression level of XIAP has been shown for many cancer types and is associated with cancer cell migration. Like most E3 ligases, XIAP ubiquitinates itself.The XIAP intrachain TR-FRET Assay Kit is a sensitive high-throughput screening (HTS) TR-FRET Assay Kit, designed to measure XIAP auto-ubiquitination activity in a homogeneous 384 reaction format. It utilizes a Europium cryptate-labeled Ub (donor) as well as Cy5-labeled Ub (acceptor) to complete the TR-FRET pairing. Since both the TR-FRET donor and acceptor are incorporated into poly-ubiquitin chains formed on XIAP, this FRET-based assay requires no time-consuming washing steps, making it especially suitable for HTS applications as well as real-time kinetics analyses of polyubiquitination.

2227.5 €

78428

NEDD4 intrachain TR-FRET Assay Kit

The NEDD4 intrachain TR-FRET Assay Kit is a sensitive high-throughput screening (HTS) TR-FRET Assay Kit, designed to measure NEDD4 auto-ubiquitination activity in a homogeneous 384 reaction format. It utilizes a Europium-labeled ubiquitin (Ub) donor as well as Cy5-labeled Ub acceptor to complete the TR-FRET pairing. Since both the TR-FRET donor and acceptor are incorporated into poly-ubiquitin chains formed on NEDD4, this assay measures poly-ubiquitination. As a homogeneous assay, it requires no time-consuming washing steps, making it especially suitable for HTS applications as well as real-time analyses.

3075 €

78543

DCAF15 Intrachain TR-FRET Assay Kit

The DCAF15 Intrachain TR-FRET Assay Kit is a sensitive high-throughput screening (HTS) TR-FRET Assay Kit designed to measure DCAF15 auto-ubiquitination activity in a homogeneous 384-reaction format. It utilizes Europium-labeled Ubiquitin (donor) and Cy5-labeled Ubiquitin (acceptor) to complete the TR-FRET pairing. This assay measures poly-ubiquitination since both the TR-FRET donor and acceptor are incorporated into poly-ubiquitin chains formed on DCAF15. As a homogenous assay, it requires no time-consuming washing steps, making it especially suitable for HTS applications as well as real-time kinetics analyses.

2475 €

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