ABC-FC0055

GFP-Mouse Heart Microvascular Endothelial Cells

Mouse Heart Microvascular Endothelial cells (mHtMECs) are isolated from hearts of 3-week old male C57BL/6 mice and GFP expressing mHtMECs-GFP were selected from puromycin resistant cells after transfected with lentiviral expressing GFP. Cells are shipped in a frozen vial (provided @ passage 3, with 5 x 105 cells/vial). mHtMECs-GFP are cultured with specially formulated Endothelial Growth Medium. Since the cells have limited proliferation capacity (cells may be expanded for a maximum of 2-3 passages) in vitro, long term culture of mHtMECs is not recommended, even cultured following the detailed protocol described below.

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cAP-m0006-SV40

SV40-Mouse Heart Microvascular Endothelial Cells

1651.35 €

ABI-TC4183

SV40-GFP-Mouse Heart Microvascular Endothelial Cells

Mouse Heart Microvascular Endothelial Cells (mHtMVECs) were initially isolated from mouse cardiac ventricle tissues of 3-week old C57/Black6 mice and SV40-GFP-mHtMVECs were selected from mHtMVECs infected with SV40 and GFP expressing lentiviruses with puromycin. Endothelial Growth Medium is recommended for the expansion of Sv40-GFP-mHtMVECs. And we warranty a minimum of 30 passages of the cells if cultured following the detailed protocol described below.

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ABC-TC4081

Rat Bladder Microvascular Endothelial Cells

Rat Bladder Microvascular Endothelial Cells from Gentaur are isolated from tissue of 7-day-old neonatal Sprague-Dawley Rats. Rat Bladder Microvascular Endothelial Cells are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and are delivered frozen.Rat Bladder Microvascular Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation, or transendothelial resistance (TER). Standard biochemical procedures performed with endothelial cell cultures include PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

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ABC-TC4096

Rat brain microvascular endothelial cells

Rat primary brain microvascular endothelial cells (RPBECs) are isolated from tissue of 1-day-old neonatal Sprague-Dawley rats. RPBECs are grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 1 hour and incubated in Culture Complete Growth Medium.

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ABC-TC4108

Rat Cardiac Microvascular Endothelial Cells

Rat Cardiac Microvascular Endothelial Cells (RCMEC) are isolated from adult Sprague Dawley rat heart tissue. RCMEC are cryopreserved from passage one culture. RCMEC are guaranteed to further expand for 10 population doublings at the conditions recommended by Gentaur.

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