SV40-GFP-Mouse Pulmonary Microvascular Endothelial Cells
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Catalog number: 870 - ABI-TC4182
Product Category: Business & Industrial > Science & Laboratory
Gentaur
Size: 1 vial
ABC-FC0053
GFP-Mouse Pulmonary Microvascular Endothelial Cells
Mouse Liver Microvascular Endothelial cells (mLivMECs) are isolated from livers of 3-week old male C57BL/6 mice and GFP expressing mLivMECs-GFP were selected from puromycin resistant cells after transfected with lentiviral expressing GFP. Cells are shipped in a frozen vial (provided @ passage 3, with 5 x 105cells/vial). mLivMECs-GFP are cultured with specially formulated Endothelial Growth Medium. Since the cells have limited proliferation capacity (cells may be expanded for a maximum of 2-3 passages) in vitro, long term culture of mLivMECs is not recommended, even cultured following the detailed protocol described below).
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ABI-TC4182
SV40-GFP-Mouse Pulmonary Microvascular Endothelial Cells
Mouse Pulmonary Microvascular Endothelial Cells (mPulMVECs) were initially isolated from mouse cardiac ventricle tissues of 3-week old C57/Black6 mice and SV40-GFP-mPulMVECs were selected from mPulMVECs infected with SV40 and GFP expressing lentiviruses with puromycin. Endothelial Growth Medium is recommended for the expansion of SV40-GFP-mPulMVECs. And we warranty a minimum of 30 passages of the cells ifcultured following the detailed protocol described below.
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