Catalog number: 535 - cAP-m0006-SV40-GFP
Product Category: Business & Industrial > Science & Laboratory
Size: 1Frozen Vial
ABC-FC0055
Mouse Heart Microvascular Endothelial cells (mHtMECs) are isolated from hearts of 3-week old male C57BL/6 mice and GFP expressing mHtMECs-GFP were selected from puromycin resistant cells after transfected with lentiviral expressing GFP. Cells are shipped in a frozen vial (provided @ passage 3, with 5 x 105 cells/vial). mHtMECs-GFP are cultured with specially formulated Endothelial Growth Medium. Since the cells have limited proliferation capacity (cells may be expanded for a maximum of 2-3 passages) in vitro, long term culture of mHtMECs is not recommended, even cultured following the detailed protocol described below.
ABC-FC0009
GFP-HDMVECs were selected from HDMVECs resistant to puromycin after infected with GFP expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 3). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels 18 when cultured following the detailed protocol described below.
ABC-FC0012
GFP-HPaMVECs were selected from HPaMVECs resistant to puromycin after infected with GFP expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 5). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels 14 when cultured following the detailed protocol described below.
ABC-FC0013
GFP-HRMVECs were selected from HRMVECs resistant to puromycin after infected with GFP expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 5). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels 16 when cultured following the detailed protocol described below.