Katalognummer: 870 - ABC-TC4241
Produktkategori: Företag och industri > Vetenskap och laboratorium
Storlek: 1 vial
ABC-TC4241
Rat Vein Smooth Muscle Cells from Gentaur are isolated from tissue of Sprague-Dawley Rats. Rat Vein Smooth Muscle Cells are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma and are characterized by immunofluorescent staining with antibodies to α-smooth muscle actin. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Gentaur.Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with smooth muscle cell cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.
ABC-TC4207
Rat Pulmonary Vein Smooth Muscle Cells from Gentaur are isolated from tissue of Sprague-Dawley Rats. Rat Pulmonary Vein Smooth Muscle Cells are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma and are characterized by immunofluorescent staining with antibodies to α-smooth muscle actin. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Gentaur.Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with smooth muscle cell cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.
ABC-TC3857
Vascular smooth muscle cell is the cellular substate of most significant arterial disease. The increased growth potential of vascular smooth muscle cells represents one of the crucial anomalies responsible for the development of essential vascular diseases. New studies demonstrate that vascular smooth muscle cells express ICAM-1 and VCAM-1, which may contribute to the inflammatory reaction in the vascular wall and may actively be involved in the progression and stability of vascular disease. In vitro culture of human VSMC as a model of vascular research played a critical role and continues providing information in the pharmacology and the therapy of vascular diseases.HUVSMC from Gentaur Research Laboratories are isolated from human umbilical veins. HUVSMC are cryopreserved at the end of secondary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HUVSMC are characterized by immunofluorescent method with antibodies to ?-smooth muscle actin and desmin. HUVSMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HUVSMC are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories.
ABC-TC3125
Bovine large artery smooth muscle cells are derived from tunica intima and tunica media of healthy, fibrous, plaque-free bovine large arteries. They are cryopreserved at second passage and can be cultured and propagated at least 16 population doublings.
ABC-TC3264
Canine Aortic Smooth Muscle Cells (CnAOSMC) are derived from tunica intima and tunica media of normal canine aorta. They are cryopreserved at second passage and can be cultured and propagated at least 10 population doublings.
ABC-TC3334
Chicken Aortic Smooth Muscle Cells (CAOSMC) are primary cells derived from fibrous-plaque-free thoracic aorta of adult chicken. They are cryopreserved at second passage and can be propagated at least 12 population doublings.