Rat Tracheal Epithelial Cells
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Catalog number: 870 - ABC-TC4233
Product Category: Business & Industrial > Science & Laboratory
Gentaur
Size: 1 vial
ABC-TC3839
Human Tracheal Epithelial Cells
The epithelium is the interface between the body and the external environment and covers all exterior surfaces and interior lumina. Depending on the tissue of origin, the functions of epithelial cells are diverse and include absorption, secretion, protection, transcellular transport, and sensation.Gentaur offers a range of Epithelia Cells produced at Gentaur's cell culture facility. The cells are isolated from normal human adult tissues of the airways (nasal mucosa, trachea, bronchi,and distal respiratory tract), breast (mammary gland), kidney (whole kidney and renal cortex), and the placenta (amniotic membrane).Shortly after isolation, all Gentaur Human Epithelial Cells are cryopreserved at passage 2 (P2) using Gentaur's proprietary, serum-free freezing medium,Cryo-SFM. Each cryo vial contains more than 500,000 viable cells after thawing.Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at Gentaur.
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ABC-TC4233
Rat Tracheal Epithelial Cells
Rat Primary Tracheal Epithelial Cells from Gentaur are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats. Rat Primary Tracheal Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Primary Tracheal Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin or ZO-1. Rat Primary Tracheal Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.
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ABC-TC3448
Human Bronchial/Tracheal Epithelial Cells
Human Bronchial/Tracheal Epithelial Cells, when grown in LIBro Medium, provide an ideal serum-free culture model for the accurate studies of toxicity, cystic fibrosis, asthma, pathogenesis, pharmacology or airway wound healing. HBTEC are cultured without retinoic acid and cryopreserved as primary cells to ensure the highest viability and plating efficiency. Our HBTEC are quality tested in LIBro B/T Medium to ensure optimal growth over a period of at least 15 population doublings at rates equal to or greater than other commercially available media. Cell Features:HBTEC are cryopreserved as primary cells. Cells are isolated from human bronchi/trachea and expanded once in culture vessels before being harvested for cryopreservation.HSAEC are cryopreserved after primary cells are isolated from human lung tissue and expanded once in culture vessels before being harvested for cryopreservation. Human Airway Epithelial Cells are not exposed to retinoic acid during isolation or expansion.Human Airway Epithelial Cells are extensively tested for quality and optimal performance.Gentaur guarantees performance and quality.
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ABC-TC4367
Human Bladder Epithelial Cells
Human Primary Bladder Epithelial Cells from Gentaur are isolated from normal human bladder tissue. Human Primary Bladder Epithelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Gentaurandrsquo; Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5×10^6 cells per ml. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. Cells are incubated with EpCAM-1 (CD326) antibody, following the application of magnetic beads pre-coated with secondary antibody.
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ABC-TC4601
Human Endometrial Epithelial Cells
Proliferation and Morphology: Normal morphology for 5 population doublings, minimum 70% viability when thawed from cryopreservation. Normal Human Endometrial (Uterine) Epithelial Cells provide an ideal serum-free culture model for many areas of research. These cells may be used to study cellular physiology of the reproductive tract, the cellular response to infectious agents, and other areas of research, Endometrial Epithelial Cells have been isolated, plated, and expanded in culture vessels three times before being harvested for cryopreservation to ensure the highest purity, viability and plating efficiency.
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ABC-TC5466
Human Cervical Epithelial Cells
Cervical Epithelial Cells have been isolated, plated, and expanded in culture vessels three times before being harvested for cryopreserved to ensure the highest purity, viability and plating efficiency. HCxEC are quality tested in Epithelial Cell Medium to ensure optimal morphology and growth over a period of at least 5 population doublings. Cervical Epithelial Cells are not exposed to antimicrobials or phenol red when cultured in Epithelial Cell Medium. Gentaur offers antimicrobials and phenol red, but they are not required for eukaryotic cell proliferation.