Rat Stomach Smooth Muscle Cells are isolated from tissue of Sprague-Dawley Rats. Cells are negative for bacteria, yeast, fungi, and mycoplasma and are characterized by immunofluorescent staining with antibodies to α-smooth muscle actin.
Mouse Stomach Smooth Muscle Cells from Gentaur are isolated from tissue of pathogen-free laboratory mice. Mouse Stomach Smooth Muscle Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Mouse Stomach Smooth Muscle Cells are characterized by immunofluorescent cell staining with antibodies of α-smooth muscle actin and are negative for bacteria, yeast, fungi, and mycoplasma.Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Gentaur.Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.
GFP-HuASMCs are puromycin resistant cells selected from HuASMCs isolated from normal human aorta, after infected with GFP expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 3-4). Smooth Muscle Cells (SMCs) Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels 8 (2-3 passages) when cultured following the detailed protocol described below.
Rat Stomach Smooth Muscle Cells are isolated from tissue of Sprague-Dawley Rats. Cells are negative for bacteria, yeast, fungi, and mycoplasma and are characterized by immunofluorescent staining with antibodies to α-smooth muscle actin.