Rat PDE4D siRNA

abx928083-15nmol

Rat PDE4D siRNA

698.5 €

ELI-21529r

Pde4d/ Rat Pde4d ELISA Kit

1475.84 €

79629

Rat PDE4D Assay Kit

Phosphodiesterase's (PDEs) play an important role in the dynamic regulation of cAMP and cAMP signaling. Rat PDE4D, also known as cAMP-inhibited phosphodiesterase, has been implicated in cardiovascular function and fertility.The Rat PDE4D Assay Kit is designed for identification of inhibitors of Rat PDE4D using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by Rat PDE4D to the binding agent.Phosphodiesterases catalyze the hydrolysis of the phosphodiester bond in dye-labeled cyclic monophosphates. Beads selectively bind the phosphate group in the nucleotide product. This increases the size of the nucleotide relative to unreacted cyclic monophosphate. In the polarization assay, dye molecules with absorption transition vectors parallel to the linearlypolarized excitation light are selectively excited. Dyes attached to the rapidly-rotating cyclic monophosphates will obtain random orientations and emit light with low polarization. Dyes attached to the slowly-rotating nucleotide-bead complexes will not have time to reorient and therefore will emit highly polarized light.The Rat PDE4D Assay Kit comes in a convenient 96-well format, with purified Rat PDE4D enzyme, fluorescently labeled substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions. The key to the Rat PDE4D Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for Rat PDE4D reactions. First, the fluorescently labeled cAMP is incubated with a sample containing Rat PDE4D for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization.

787.5 €

60075

Rat PDE7B, GST-tag

Rat PDE7B (GenBank Accession No. NM_080894), a.a. 108-446(end), with N-terminal GST-tag, MW=65 kDa, expressed in a Baculovirus-infected Sf9 cell expression system.

832.5 €

79571

Rat PDE4B Assay Kit

Phosphodiesterases (PDEs) play an important role in dynamic regulation of cAMP and cGMP signaling. PDE4 selective inhibitors are currently in clinical trials for the treatment of diseases related to inflammatory disorders. Altered activity of PDE4B has been associated with schizophrenia and bipolar disorder. It has also recently been found to modulate cognition, as reduction in PDE4B activity improves memory and long-term plasticity in mouse models._x000D_The Rat PDE4B Assay Kit is designed for identification of inhibitors of rat PDE4B (rPDE4B) using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by rPDE4B to the binding agent._x000D_<p class="title1">Phosphodiesterases catalyze the hydrolysis of the phosphodiester bond in dye-labeled cyclic monophosphates. Beads selectively bind the phosphate group in the nucleotide product. This increases the size of the nucleotide relative to unreacted cyclic monophosphate. In the polarization assay, dye molecules with absorption transition vectors parallel to the linearly-polarized excitation light are selectively excited. Dyes attached to the rapidly-rotating cyclic monophosphates will obtain random orientations and emit light with low polarization. Dyes attached to the slowly-rotating nucleotide-bead complexes will not have time to reorient and therefore will emit highly polarized light._x000D_The Rat PDE4B Assay Kit comes in a convenient 96-well format, with purified rPDE4B enzyme, fluorescently labeled rPDE4B substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions. The key to the Rat PDE4B Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for rPDE4B reactions. First, the fluorescently labeled cAMP is incubated with a sample containing rPDE4B for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization.

757.5 €

79634

Rat PDE7A Assay Kit

Phosphodiesterases (PDEs) play an important role in the dynamic regulation of cAMP and cAMP signaling. Rat PDE7A, also known as high affinity cAMP-specific phosphodiesterase 7A, has been implicated in cardiovascular function and fertility. The Rat PDE7A Assay Kit is designed for identification of inhibitors of Rat PDE7A using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by Rat PDE7A to the binding agent. Phosphodiesterases catalyze the hydrolysis of the phosphodiester bond in dye-labeled cyclic monophosphates. Beads selectively bind the phosphate group in the nucleotide product. This increases the size of the nucleotide relative to unreacted cyclic monophosphate. In the polarization assay, dye molecules with absorption transition vectors parallel to the linearly-polarized excitation light are selectively excited. Dyes attached to the rapidly-rotating cyclic monophosphates will obtain random orientations and emit light with low polarization. Dyes attached to the slowly-rotating nucleotide-bead complexes will not have time to reorient and therefore will emit highly polarized light.The Rat PDE7A Assay Kit comes in a convenient 96-well format, with purified Rat PDE7A enzyme, fluorescently labeled substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions. The key to the Rat PDE7A Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for Rat PDE7A reactions. First, the fluorescently labeled cAMP is incubated with a sample containing Rat PDE7A for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization.

757.5 €