Rat Kidney Proximal Tubular Epithelial Cells
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Catalog number: 870 - ABC-TC4149
Product Category: Business & Industrial > Science & Laboratory
Gentaur
Size: 1 vial
ABC-TC4149
Rat Kidney Proximal Tubular Epithelial Cells
Rat Kidney Proximal Tubular Epithelial Cells from Gentaur are isolated from tissue of 5-day-old neonatal laboratory Sprague–Dawley Rats. Rat Kidney Proximal Tubular Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Kidney Proximal Tubular Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin or ZO-1. Rat Kidney Proximal Tubular Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.
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ABC-TC5532
Rat Renal Proximal Tubular Epithelial Cells
Renal proximal tubular epithelial cells (RPTEpiC) play a crucial role in renal function. They reabsorb nearly all of the glucose and amino acids in the glomerular filtrate, while allowing other substances of no nutritional value to be excreted in the urine. They are also a major site of injury in a variety of congenital, metabolic, and inflammatory diseases. RPTEpiC can produce inflammatory mediators such as cytokines or chemokines and actively participate in acute inflammatory processes by affecting and directing leukocyte chemotaxis via the production of IL-8. RPTEpiC express IL-2R alpha and MHC class II antigens during inflammation after renal transplantation or in crescentic glomerulonephritis, indicating the capacity to participate in the pathogenesis of immune renal injury. To be able to study the relationship between proximal tubular cells and a variety of renal diseases, the RPTEpiC culture provides a useful in vitro model.
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