Rat Colonic Smooth Muscle Cells
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Catalog number: 870 - ABC-TC4114
Product Category: Business & Industrial > Science & Laboratory
Gentaur
Size: 1 vial
ABC-TC3572
Human Colonic Smooth Muscle Cells
Smooth muscle contraction is the fundamental event in gastrointestinal motion. Inflammation of the human intestine causes thickening of the smooth muscle layers which results from the increases in the smooth muscle-specific actins. The increased smooth muscle actins may affect force production and further demonstrate the plasticity of smooth muscle in the inflamed intestine. Human intestinal smooth muscle cells respond to IL-1-beta and TNF-alpha stimulation by releasing IL-6, which might significantly contribute to the overall systemic inflammatory response. The colonic smooth muscle cells possess a different subtype of somatostatin receptor from gastric smooth muscle cells. The availability of human gastrointestinal smooth muscle cells in culture will considerably enhance our ability to study the contractile, proliferative and connective tissue responses of the smooth muscle of the human gastrointestinal tract.HCSMC from Gentaur Research Laboratories are isolated from the human colon. HCSMC are cryopreserved at primary or passage one culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HCSMC are characterized by immunofluorescent method with antibodies to andALPHA;-smooth muscle actin and desmin. HCSMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCSMC are guaranteed to further expand for 15 population doublings in the condition provided by Gentaur Research Laboratories.
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ABC-TC4114
Rat Colonic Smooth Muscle Cells
Rat Colonic Smooth Muscle Cells from Gentaur are isolated from tissue of Sprague-Dawley Rats. Rat Colonic Smooth Muscle Cells are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma and are characterized by immunofluorescent staining with antibodies to α-smooth muscle actin. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Gentaur.Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with smooth muscle cell cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.
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