Catalog number: 870 - ABC-TC4082
Product Category: Business & Industrial > Science & Laboratory
Size: 1 vial
ABC-TC4082
Rat bladder smooth muscle cells, 3-week Wistar rat
ABC-TC3514
Smooth muscle tissue is found in the tunica media layer of large and small blood vessels and in the walls of hollow organs like the bladder and the uterus. Smooth Muscle Cells (SMC) possess all the same types of filaments, but, depending on the tissue of origin, differ significantly in mechanical and physiological properties. Therefore, Gentaur offers a range of Human Smooth Muscle Cells produced at Gentaur's cell culture facility from normal human tissue of different origins. The cells are isolated from the aorta, the coronary artery, the pulmonary artery, the umbilical artery,the trachea, the bronchi, the bladder,the prostate, and the uterus. Shortly after isolation, all Gentaur Human Smooth Muscle Cells are cryopreserved at passage 2 (P2) using Gentaur's proprietary, serum-free freezing medium, Cryo-SFM. Each cryo vial contains more than 500,000 viable cells after thawing.Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at Gentaur.
ABC-TC4235
Rat Tracheal Smooth Muscle Cells from Gentaur are isolated from tissue of Sprague-Dawley Rats. Rat Tracheal Smooth Muscle Cells are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma and are characterized by immunofluorescent staining with antibodies to α-smooth muscle actin. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Gentaur.Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with smooth muscle cell cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.
ABC-TC4241
Rat Vein Smooth Muscle Cells from Gentaur are isolated from tissue of Sprague-Dawley Rats. Rat Vein Smooth Muscle Cells are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma and are characterized by immunofluorescent staining with antibodies to α-smooth muscle actin. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Gentaur.Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with smooth muscle cell cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.