Catalog number: 870 - ABC-TC4078
Product Category: Business & Industrial > Science & Laboratory
Size: 1 vial
ABC-TC3913
Different parts of the C57 mouse brain, including the cortex, hippocampus, and cerebellum, are dissociated enzymatically and mechanically into suspension of single cells. Next, the cells are seeded, and the astrocytes are enriched in vitro, using specific medium and cultural conditions.
ABC-TC4078
Astrocytes are the majority cell type of the mammalian brain. Astrocytes have been implicated in a variety of supportive functions for their partner neurons in the CNS, such as neuronal guidance during development
ABC-TC3505
Astrocytes make up the majority of the cells in the mammalian brain. They are the most variable in type, most intimately associated with all parts of neurons, and thus most functionally interesting in their relationships with neurons. They provide structural, trophic, and metabolic support to neurons and modulate synaptic activity. Impairment of these astrocyte functions during stroke and other insults can critically influence neuron survival. Furthermore, astrocytes have been implicated in the pathological processes of many neurological diseases. Long-term recovery after brain injury, through neurite outgrowth, synaptic plasticity, or neuron regeneration, is influenced by astrocyte surface molecule expression and trophic factor release. In addition, the death or survival of astrocytes themselves may affect the ultimate clinical outcome. Recognition of the importance of astrocytes in nervous system functioning is increasing, specifically regarding the modulation of neural activity. Much of what we have learned about astrocytes is from the in vitro studies and astrocyte cultures are continuing to provide a useful tool in exploring the diverse property of these cells.HA-c from Gentaur Research Laboratories are isolated from human cerebellar. HA-c are cryopreserved at passage one and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HA-c are characterized by immunofluorescent method with antibody to GFAP. HA-c are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HA-c are guaranteed to further expand for 15 population doublings at the conditions provided by Gentaur Research Laboratories.
XBL-10133
Human cerebellar peduncles tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human cerebellar peduncles tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the cerebellar peduncles tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The cerebellar peduncles tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.